76043abf2ebed2c

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران

2 دانشگاه رازی

چکیده

بیماری زردی و پژمردگی فوزاریومی نخود با عوامل قارچی Fusarium redolens (به اختصار: Fr) و Fusarium oxysporum f. sp. ciceris (به اختصار: Foc)، از بیماری‌های مهم این محصول در ایران است. در این پژوهش اثر برخی سویه‌های پروبیوتیک باکتریایی و قارچی دریافتی از منابع مختلف که متعلق به جنس‌های Alcaligenes، Bacillus، Delftia، Pseudomonas، Stenotrophomonas و Trichoderma بودند، در مهار بیمارگرهای مذکور در آزمایشگاه و گلخانه و تأثیرشان بر شاخص‌های رشدی نخود در حضور بیمارگرها بررسی شد. ترکیبات فرّار کلیه پروبیوتیک‌ها از رشد میسلیوم بیمارگرها جلوگیری نمودند. در آزمون کشت متقابل نیز همه پروبیوتیک‌ها بر رشد Foc اثر بازدارنده داشتند، اما فقط اثر Bacillus pumilus INR7 و Trichoderma harzianum T33 در مهار رشد Fr معنی‌دار بود. در گلخانه، Delftia tsuruhatensis PIIR و1624 Alcaligenes faecalis به ترتیب با کاهش 38٫۲ و 23٫58 درصدی زردی و 35٫2 درصدی نکروز ریشه ناشی از Fr و افزایش فاکتورهای رشدی، و در مقابل، T. harzianum T33 و Pseudomonas putida RUP1 به ترتیب با کاهش 50 و ۶۶/۳۷ درصدی زردی و ۷۵/۴۴ و ۸۴/۳۷ درصدی نکروز آوندی ناشی از Foc و بهبود رشد نخود، جزو بهترین تیمارها بودند؛ در مجموع، پروبیوتیک‌های مورد استفاده، پتانسیل تحریک رشد گیاه و بیوکنترل عوامل زردی و پژمردگی فوزاریومی نخود را داشتند.

کلیدواژه‌ها

Afzal I, Shinwari ZK, Sikandar S, Shahzad S (2019) Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiological Research 221: 36-49.
Ardalan A, Abbasi S, Sharifi R (2017) Effect of some mineral elements on biocontrol efficiency of Bacillus pumilus INR7 against bean damping-off caused by Rhizoctonia solani. Biological Control of Pests and Plant Diseases 6(2): 187-195 (In Persian).
Arora D, Pandey A (1989) Effects of soil solarization on Fusarium wilt of chickpea. Journal of Phytopathology 124(1): 13-22.
Ben Abdallah RA, Jabnoun-Khiareddine H, Nefzi A, Mokni-Tlili S, Daami-Remadi M (2016) Endophytic bacteria from Datura stramonium for Fusarium wilt suppression and tomato growth promotion. Journal of Microbial and Biochemical Technology 8(1): 030-041.
Ben Abdallah RA, Mejdoub-Trabelsi BM (2016) Isolation of endophytic bacteria from Withania somnifera and assessment of their ability to suppress Fusarium wilt disease in tomato and to promote plant growth. Journal of Plant Pathology & Microbiology 07(05).
Benítez T, Rincón AM, Limón MC, Codon AC (2004) Biocontrol mechanisms of Trichoderma strains. International Microbiology 7(4): 249-260.
Berg G, Roskot N, Smalla K (1999) Genotypic and phenotypic relationships between clinical and environmental isolates of Stenotrophomonas maltophilia. Journal of Clinical Microbiology 37(11): 3594-3600.
Bhattacharyya PN, Jha DK (2012) Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology 28(4): 1327-1350.
Bouhadida M, Jendoubi W, Gargouri S, Beji M, Kharrat M, Chen W (2017) First report of Fusarium redolens causing Fusarium yellowing and wilt of chickpea in Tunisia. Plant Disease 101(6): 1038-1038.
Contreras-Cornejo HA, Macias-Rodriguez L, Beltran-Pena E, Herrera-Estrella A, Lopez-Bucio J (2011) Trichoderma-induced plant immunity likely involves both hormonal- and camalexin-dependent mechanisms in Arabidopsis thaliana and confers resistance against necrotrophic fungi Botrytis cinerea. Plant Signaling and Behavior 6(10): 1554-63.
Costa JM, Loper JE (1994) Characterization of siderophore production by the biological control agent Enterobacter cloacae. Molecular Plant Microbe Interactions 7(4): 440-448.
Dennis C, Webster J (1971) Antagonistic properties of species-groups of Trichoderma: I. Production of non-volatile antibiotics. Transactions of the British Mycological Society 57(1): 41-48.
Dubey SC, Suresh M, Singh B (2007) Evaluation of Trichoderma species against Fusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt. Biological Control 40(1): 118-127.
Duke KA, Becker MG, Girard IJ, Millar JL, Dilantha Fernando WG, Belmonte MF, de Kievit TR (2017) The biocontrol agent Pseudomonas chlororaphis PA23 primes Brassica napus defenses through distinct gene networks. BMC Genomics 18(1): 467.
Ebrahimi Kazemabad Z, Rohani H, Jamali F, Mahdikhani Moghadam E (2012) Antagonistic effect of Pseudomonas fluorescens isolates against Fusarium oxysporum f. sp. ciceris. Iranian Journal of Pulses Research 3(2): 55-64 (In Persian).
Elmahdi S, Kadir J, Mohamed MTM, Vadamalai G, Akter S (2015) Isolation, screening and characterization of effective microbes with Potential for biological control of Fusarium wilt of rock melon. World Journal of Agricultural Research 3(1): 11-16.
Elshahat MR, Ahmed AA, Enas AH, Fekria MS (2016) Plant growth promoting rhizobacteria and their potential for biocontrol of phytopathogens. African Journal of Microbiology Research 10(15): 486-504.
Esmaeili Taheri A, Hamel C, Gan Y, Vujanovic V (2011) First report of Fusarium redolens from Saskatchewan and its comparative pathogenicity. Canadian Journal of Plant Pathology 33(4): 559-564.
Garbeva P, Van Elsas J, Van Veen J (2008) Rhizosphere microbial community and its response to plant species and soil history. Plant and Soil 302(1-2): 19-32.
Ghanbarzadeh B, Safaie N, Goltapeh EM (2014) Antagonistic activity and hyphal interactions of Trichoderma spp. against Fusarium proliferatum and F. oxysporum In vitro. Archives of Phytopathology and Plant Protection 47(16): 1979-1987.
Ghasemi A, Rostami M, Yazdaninia T (2012) In vitro antagonistic and biodegradation activity of a newly isolated Delftia tsuruhatensis from rice plant in Iran. International Journal of Agriculture and Crop Sciences 4(2): 40-44.
Glazebrook J (2005) Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annual Review of Phytopathology 43(1): 205-27.
Gong AD, Wu NN, Kong XW, Zhang YM, Hu MJ, Gong SJ, Dong FY, Wang JH, Zhao ZY, Liao YC (2019) Inhibitory effect of volatiles emitted from Alcaligenes faecalis N1-4 on Aspergillus flavus and aflatoxins in storage. Frontiers in Microbiology 10: 1419.
Han J, Sun L, Dong X, Cai Z, Sun X, Yang H, Wang Y, Song W (2005) Characterization of a novel plant growth-promoting bacteria strain Delftia tsuruhatensis HR4 both as a diazotroph and a potential biocontrol agent against various plant pathogens. Systematic and Applied Microbiology 28(1): 66-76.
Harvás A, Landa B, Jiménez-Díaz RM (1997) Influence of chickpea genotype and Bacillus sp. on protection from Fusarium wilt by seed treatment with nonpathogenic Fusarium oxysporum. European Journal of Plant Pathology 103(7): 631-642.
Heil M (2008) Indirect defence via tritrophic interactions. New Phytologist 178(1): 41-61.
Herrera-Tellez VI, Cruz-Olmedo AK, Plasencia J, Gavilanes-Ruiz M, Arce-Cervantes O, Hernandez-Leon S, Saucedo-Garcia M (2019) The protective effect of Trichoderma asperellum on tomato plants against Fusarium oxysporum and Botrytis cinerea diseases involves inhibition of reactive oxygen species production. International Journal of Molecular Sciences 20(8).
Huang Y, He Y, Ye B-C, Li C (2017) Rhizospheric Bacillus subtilis exhibits biocontrol effect against Rhizoctonia solani in pepper (Capsicum annuum). BioMed Research International 2017: 1-9.
Hwang SF, Howard RJ, Chang KF, Park B, Burnett PA (1994) Etiology and severity of fusarium root rot of lentil in Alberta. Canadian Journal of Plant Pathology 16(4): 295-303.
Inbar J, Abramsky M, Cohen D, Chet I (1994) Plant growth enhancement and disease control by Trichoderma harzianum in vegetable seedlings grown under commercial conditions. European Journal of Plant Pathology 100(5): 337-346.
Jamali F, Sharifi Tehrani A, Okhovvat M, Zakeri Z (2005) Effect of antagonistic bacteria on the control of Fusarium wilt of chickpea caused by Fusarium oxysporum. Iranian, J. Agric. Sci. 36: 711-717 (In Persian).
Janahiraman V, Anandham R, Kwon SW, Sundaram S, Karthik Pandi V, Krishnamoorthy R, Kim K, Samaddar S, Sa T (2016) Control of wilt and rot pathogens of tomato by antagonistic pink pigmented facultative methylotrophic Delftia lacustris and Bacillus spp. Front Plant Sci 7: 1626.
Jasem AM, Sharifi R, Abbasi S (2018) Induced systemic resistance to wheat take-all disease by probiotic bacteria. Journal of Plant Protection Research 58(3): 304-310.
Jendoubi W, Bouhadida M, Boukteb A, Béji M, Kharrat M (2017) Fusarium wilt affecting chickpea crop. Agriculture 7(3): 23.
Jeong DE, Lee SJ, Seul KJ, Park YM, Ghim SY (2006) Characterization of diazotrophs isolated from rice rhizosphere and their antifungal activities. Microbiology and Biotechnology Letters 34(2): 180-184.
Jeong H, Choi SK, Kloepper JW, Ryu CM (2014) Genome sequence of the plant endophyte Bacillus pumilus INR7, triggering induced systemic resistance in field crops. Genome Announcements 2(5): e01093-14.
Jimenez-Fernandez D, Navas-Cortes JA, Montes-Borrego M, Jimenez-Diaz RM, Landa BB (2011) Molecular and pathogenic characterization of Fusarium redolens, a new causal agent of Fusarium yellows in chickpea. Plant Disease 95(7): 860-870.
Jimenez-Fernandez D, Landa BB, Kang S, Jimenez-Diaz RM, Navas-Cortes JA (2013) Quantitative and microscopic assessment of compatible and incompatible interactions between chickpea cultivars and Fusarium oxysporum f. sp. ciceris races. PloS One 8(4): e61360.
Jogaiah S, Abdelrahman M, Tran LSP, Ito SI (2018) Different mechanisms of Trichoderma virens-mediated resistance in tomato against Fusarium wilt involve the jasmonic and salicylic acid pathways. Molecular Plant Pathology 19(4): 870-882.
Jørgensen NO, Brandt KK, Nybroe O, Hansen M (2009) Delftia lacustris sp. nov., a peptidoglycan-degrading bacterium from fresh water, and emended description of Delftia tsuruhatensis as a peptidoglycan-degrading bacterium. International Journal of Systematic and Evolutionary Microbiology 59(9): 2195-2199.
Kaur R, Singh R, Alabouvette C (2007) Antagonistic activity of selected isolates of fluorescent Pseudomonas against Fusarium oxysporum f. sp. ciceri. Asian Journal of Plant Sciences 6(3): 446-454.
Kloepper JW, Ryu CM, Zhang SA (2004) Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94(11): 1259-1266.
Knudsen I, Hockenhull J, Jensen DF, Gerhardson B, Hökeberg M, Tahvonen R, Teperi E, Sundheim L, Henriksen B (1997) Selection of biological control agents for controlling soil and seed-borne diseases in the field. European Journal of Plant Pathology 103(9): 775-784.
Köhl J, Kolnaar R, Ravensberg WJ (2019) Mode of action of microbial biological control agents against plant diseases: Relevance beyond efficacy. Frontiers in Plant Science 10: 845.
Kumari P, Khanna V (2019) Seed bacterization stimulated resistance in chickpea against Fusarium oxysporum f. sp. ciceris. Indian Phytopathology 72(4): 689-697.
Landa BB, Navas-Cortes JA, Jimenez-Diaz RM (2004) Integrated management of fusarium wilt of chickpea with sowing date, host resistance, and biological control. Phytopathology 94(9): 946-60.
Lemanceau P, Alabouvette C (1993) Suppression of fusarium wilts by fluorescent pseudomonads: Mechanisms and applications. Biocontrol Science and Technology 3(3): 219-234.
Lokesh S, Bharath B, Raghavendra V, Govindappa M (2007) Importance of plant growth-promoting rhizobacteria in enhancing the seed germination and growth of watermelon attacked by fungal pathogens. Acta Agronomica Hungarica 55(2): 243-249.
Lorito M, Hayes C, Di Pietro A, Woo S, Harman GJP (1994) Purification, characterization, and synergistic activity of a glucan 1, 3-beta-glucosidase and an N-acetyl-beta-glucosaminidase from Trichoderma harzianum. Phytopathology 84(4): 398-405.
MartínezMedina A, Fernandez I, Lok GB, Pozo MJ, Pieterse CM, Van Wees S (2017) Shifting from priming of salicylic acid‐to jasmonic acid‐regulated defences by Trichoderma protects tomato against the root knot nematode Meloidogyne incognita. New Phytologist 213(3): 1363-1377.
Minkwitz A, Berg G (2001) Comparison of antifungal activities and 16S ribosomal DNA sequences of clinical and environmental isolates of Stenotrophomonas maltophilia. Journal of Clinical Microbiology 39(1): 139-145.
Morel MA, Cagide C, Minteguiaga MA, Dardanelli MS, Castro-Sowinski S (2015) The pattern of secreted molecules during the co-inoculation of alfalfa plants with Sinorhizobium meliloti and Delftia sp. strain JD2: an interaction that improves plant yield. Molecular Plant-Microbe Interactions 28(2): 134-142.
Nautiyal CS (1997) Selection of chickpea-rhizosphere-competent Pseudomonas fluorescens NBRI1303 antagonistic to Fusarium oxysporum f. sp. ciceri, Rhizoctonia bataticola and Pythium sp. Current Microbiology 35(1): 52-58.
Nwankiti A, Gwa V (2018) Evaluation of antagonistic effect of Trichoderma harzianum against Fusarium oxysporum causal agent of white yam (Dioscorearotundata poir) tuber rot. Trends in Technical & Scientific Research 1(1): 0012-0018.
Palmieri D, Vitullo D, De Curtis F, Lima G (2016) A microbial consortium in the rhizosphere as a new biocontrol approach against fusarium decline of chickpea. Plant and Soil 412(1-2): 425-439.
Pieterse CM, Van der Does D, Zamioudis C, Leon-Reyes A, Van Wees SC (2012) Hormonal modulation of plant immunity. Annual Review of Cell and Developmental Biology 28: 489-521.
Prasannakumar SP, Gowtham HG, Hariprasad P, Shivaprasad K, Niranjana SR (2015) Delftia tsuruhatensis WGR-UOM-BT1, a novel rhizobacterium with PGPR properties from Rauwolfia serpentina (L.) Benth. ex Kurz also suppresses fungal phytopathogens by producing a new antibiotic-AMTM. Letters in Applied Microbiology 61(5): 460-8.
Sayyed RZ, Chincholkar SB (2009) Siderophore-producing Alcaligenes feacalis exhibited more biocontrol potential vis-à-vis chemical fungicide. Current Microbiology 58(1): 47-51.
Seifi S (2019) Screening of nitrate removal bacteria and effect of these bacteria on biological control of Fusarium wilt disease in tomato. PhD thesis, University of Tehran, Karaj, Iran.
Shabir R R, Kumar EJ, Talat M, Ganie SA, Dar WA, Bhat JA (2013) Eco-friendly management of root-rot of chilli caused by Rhizoctonia solani Kuhn. African Journal of Agricultural Research 8(21): 2563-2566.
Sharifi R, Ryu C-M (2016a) Are bacterial volatile compounds poisonous odors to a fungal pathogen Botrytis cinerea, alarm signals to Arabidopsis seedlings for eliciting induced resistance, or both? Frontiers in Microbiology 7: doi:10.3389/fmicb.2016.00196.
Sharifi R, Ryu CM (2016b) Making healthier or killing enemies? Bacterial volatile-elicited plant immunity plays major role upon protection of Arabidopsis than the direct pathogen inhibition. Communicative and Integrative Biology 9(4): e1197445.
Sharifi R, Ryu CM (2017) Chatting With a Tiny Belowground Member of the Holobiome. Advances in Botanical Research 82: 135-160.
Sharifi R, Ryu C-M (2020) Contribution of Bacterial Volatiles to Chemical Ecology, In: Ryu C-M, L Weisskopf and B Piechulla (ed.), Bacterial Volatile Compounds as Mediators of Airborne Interactions. Springer Singapore, Singapore. pp. 167-186.
Sohrabi F, Sheikholeslami M, Heydari R, Rezaee S, Sharifi R (2020) Investigating the effect of Glomus mosseae, Bacillus subtilis and Trichoderma harzianum on plant growth and controlling Meloidogyne javanica in tomato. Indian Phytopathology 73(2): 293-300.
Sohrabi M (2016) Biological control of Fusarium species associated to chickpea wilt and root rot in Kermanshah and Kurdistan Proviences by plant growth promoting rhizobacteria. MSc thesis, Razi University, Kermanshah, Iran.
Sreeramulu K, Jayalakshmi SK, Raju S, S U, Benagi V (2009) Trichoderma harzianum L1 as a potential source for lytic enzymes and elicitor of defense responses in chickpea (Cicer arietinum L.) against wilt disease caused by Fusarium oxysporum f. sp. ciceri. Australian Journal of Crop Science 3(1): 44-52.
Szentes S, Radu G-L, Laslo É, Lányi S, Mara G (2013) Selection and evaluation of potential biocontrol rhizobacteria from a raised bog environment. Crop Protection 52: 116-124.
Tekeoğlu M, Özkılınç H, Tunalı B, Küsmenoğlu İ, Chen W (2017) Molecular identification of Fusarium spp. causing wilt of chickpea and the first report of Fusarium redolens in Turkey. Medıterranean Agrıcultural Scıences 30(1): 27-33.
Turlings TCJ, Erb M (2018) Tritrophic interactions mediated by herbivore-induced plant volatiles: Mechanisms, ecological relevance, and application potential. Annual Review of Entomology 63(1): 433-452.
Vacheron J, Desbrosses G, Bouffaud ML, Touraine B, Moënne-Loccoz Y, Muller D, Legendre L, Wisniewski-Dyé F, Prigent-Combaret C (2013) Plant growth-promoting rhizobacteria and root system functioning. Frontiers in Plant Science 4: 356.
Vaja KN, Gajera HP, Hirpara DG, Vaja VN, Golakiya BA (2016) Biochemical characterization and molecular identification of Pseudomonas antagonists inhibiting Fusarium oxysporum f. sp. ciceri and Sclerotium rolfsii Sacc. Indian Journal of Agricultural Biochemistry 29(2): 175-183.
Validov SZ, Kamilova F, Lugtenberg BJ (2009) Pseudomonas putida strain PCL1760 controls tomato foot and root rot in stonewool under industrial conditions in a certified greenhouse. Biological Control 48(1): 6-11.
Villamizar-Gallardo RA, Ortíz-Rodriguez OO, Escobar JW (2017) Symbiotic and endophytic fungi as biocontrols against cocoa (Theobroma cacao L.) phytopathogens. Summa Phytopathologica 43(2): 87-93.
Wozniak M, Galazka A, Tyskiewicz R, Jaroszuk-Scisel J (2019) Endophytic bacteria potentially promote plant growth by synthesizing different metabolites and their phenotypic/physiological profiles in the biolog GEN III MicroPlateTM Test. International Journal of Molecular Sciences 20: 5283.
Zarbanoei G (2015) Genetic diversity of Fusarium oxysporum species complex, the causal agent of chickpea wilt and leaf yellowing in Kermanshah Province. M. Sc. thesis, Razi University, Kermanshah, Iran.