76043abf2ebed2c

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه گیاهپزشکی دانشگاه ولی عصر (ع) رفسنجان-

2 عضو هیات علمی گروه گیاهپزشکی دانشگاه ولی عصر رفسنجان

چکیده

باکتری‌های محرک رشد گیاهان با داشتن توان بالا در جهت افزایش زیست‌توده و کنترل بیماری‌های گیاهی، می‌توانند جانشین مناسبی برای سموم و کودهای شیمیایی در کشاورزی باشند. به‌منظور بررسی مهار زیستی Pseudomonas syringae pv. lachrymans عامل بیماری لکه زاویه‌ای خیار در شرایط آزمایشگاه و گلخانه‌، از باکتری‌های جداسازی شده از خاک ریزوسفر خیار به همراه تعدادی از نمونه‌های مجموعه باکتری‌های گروه گیاه‌پزشکی دانشگاه ولی‌عصر رفسنجان استفاده شد. بعد از غربالگری آزمایشگاهی، چهار جدایه که دارای توان ایجاد هاله بازدارنده بودند برای بررسی‌های بعدی در شرایط آزمایشگاه و گلخانه انتخاب شدند. خصوصیات مهار زیستی جدایه‌ها مانند تولید متابولیت‌های ثانویه و بعضی از ویژگی‌های تحریک‌کنندگی رشد در شرایط آزمایشگاهی مورد ارزیابی قرار گرفت. در ارزیابی‌های گلخانه‌ای، توان جدایه‌های باکتریایی در کنترل بیماری از طریق ممانعت در ایجاد علایم با تعیین سطح زیر منحنی پیشرفت بیماری و افزایش زیست‌توده گیاه خیار با بررسی شاخص‌های رشدی مطالعه شد. نتایج بررسی گلخانه‌ای نشان داد که جدایه Pseudomonas fluorescens VRPF 54 کارآیی مناسبی در مهار زیستی بیماری داشت. در شرایط گلخانه، این جدایه به تنهایی و تواًم با بیمارگر به طور معنی‌داری باعث افزایش زیست‌توده و کنترل بیماری لکه زاویه‌ای خیار گردید. پس از انتخاب این جدایه، برخی از ویژگی‌های آنتاگونیستی، خصوصیات بیوشیمیایی و شناسایی مولکولی آن در آزمایشگاه مورد بررسی قرار گرفت. همچنین نتایج حاصل از اندازه‌گیری آنزیم‌های پراکسیداز، گایاکول‌پراکسیداز، فنیل‌آلانین‌آمونیالیاز، پلی‌فنل‌اکسیداز و محتوی فنل کل روند افزایشی را نشان داد.

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