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Biocontrol potential of endophytic Penicillium spp. against strawberry anthracnose

نوع مقاله: مقاله کامل

نویسندگان

دانشگاه کردستان

چکیده

بیماری آنتراکنوز توت فرنگی به وسیله‌ی قارچ Colletotrichum nymphaeae ایجاد و یکی از مهمترین بیماریهای توت-فرنگی در استان کردستان است که خسارت قابل توجهی به این محصول وارد می‌کند. در این تحقیق تاثیر قارچ‌های اندوفیت جدا شده از ساقه و طوقه توت فرنگی روی این بیماری بررسی شد. جدایه‌های تاثیرگذار روی بیماری انتخاب و بر اساس روش‌های ریخت شناسی و مولکولی بررسی و سرانجام دو گونه Penicillium hordei و Penicillium polonicum شناسایی شدند. تاثیرات ضد قارچی هر دوگونه در شرایط آزمایشگاهی با استفاده از روش‌های کشت متقابل، تولید ترکیبات فرار و غیر فرار و شرایط گلخانه روی بیماری بررسی شد. هر دو گونه با تولید ترکیبات فرار توانستند رشد پرگنه قارچ را به میزان 05/34 تا 36/42 درصد در مقایسه با شاهد کاهش دهند. علاوه بر این دو گونه P. hordei و P. polonicum شدت بیماری آنتراکنوز توت فرنگی را به میزان 9/58 و 4/61 درصد بترتیب کاهش دادند. بنابر این، نتایج آزمایشگاهی و گلخانه‌ای نشان داد که هر دو گونه قارچ قادر هستند که این بیماری را تا حد قابل توجهی کاهش دهند و این امکان وجود دارد که گونه‌های جنس Penicillium بتوانند مدیریت بیماری آنتراکنوز توت فرنگی را فراهم کنند. بر اساس مطالعات صورت گرفته این اولین گزارش از تاثیر گونه های مربوط به Penicillium روی بیماری آنتراکنوز توت فرنگی است و لذا تحقیقات تکمیلی برای این مهم ضروری است.

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موضوعات


Arnold AE )2007( Understanding the diversity of foliar endophytic fungi: progress. Challenges, and frontiers. Fungal Biology Rev 21: 51-66.

Arnold AE, Mejia LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA (2003) Fungal endophytes limit pathogen damage in a tropical tree. Proceedings of the National Academy of Sciences of the United States of America 100: 15649-15654.

Baroncelli R, Zapparata A, Sarrocco S, Sukno SA, Lane CR, Thon MR, Vannacci G, Holub E,  Sreenivasaprasad S ( 2015) Molecular diversity of anthracnose pathogen populations associated with UK strawberry production suggests multiple introductions of three different Colletotrichum species. PloS One 10 (6): e0129140.

Benhamou N, Kloepper JW, Quadt-Hallmann A, Tuzun S (1996) Induction of defense-related ultrastructural modification in pea root tissues inoculated with endophytic bacteria. Physiology Plant Pathology 112: 919-929.

Cao Y, Zhang Z, Ling N, Yuan Y, Zheng X, Shen B, Shen Q (2011) Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots. Biology and Fertility of Soils 47 (5): 495–506.

Chalfoun NR, Castagnaro AP, Diaz Ricci JC (2011) Induced resistance activated by culture filtrate derived from an avirulent pathogen as a mechanism of biological control of anthracnose in strawberry. Biological Control 58: 319-329.

Chen Y, Gao X, Chen Y, Qin H, Huang L, Han Q (2014) Inhibitory efficacy of endophytic Bacillus subtillis EDR4 against Sclerotinia sclerotiorum on rapeseed. Biological Control 78: 67-76.

Damm U, Cannon PF, Woudenberg JHC, Crous PW (2012) The Colletotrichum acutatum species complex. Studies in Mycology 73: 37–114.

Delp B, Milholland R (1980) Evaluation strawberry plants for resistance to Colletotrichum fragariae. Plant disease 64 (12): 1071-1073.

Denoyes-Rothan B, Lafargue M, Guerin G, Clerjeau M (1999) Fruit resistance to Colletotrichum acutatum in strawberries. Plant Disease 83: 549-553.

Francesco AD, Uoglini L, Lazzeri L, Mari M (2015) Production of volatile organic compounds by Aureobasidium pullulans as a potential mechanism of action against postharvest fruit pathogens. Biological Control 81: 8-14.

Freeman S, Katan T (1997) Identification of Colletotrichum species responsible for anthracnose and root necrosis of strawberry in Israel. Phytopathology 87: 516–521.

Gao FK, Dai CC, Liu XZ (2010) Mechanisms of fungi endophytes in plant protection against pathogens. African Journal Microbiology Research 4: 1346-1351.

Houbraken J, Wang L, Lee HB, Frisvad JC (2016) New section in Penicillium containing novel species producing patulin, Pyripyropens or other bioactive compounds. Personia 36: 299-314.

Howard CM, Mass JL, Chandler CK, Albregts EE (1992) Anthracnose of strawberry caused by the Colletotrichum complex in Florida. Plant Disease 76: 976-981.

Huang R, Li GQ, Zhang J, Yang I, Che HJ, Jiang DH, Huang HC (2011) Control of postharvest Botrytis fruit rot of strawberry by volatile organic compound of Candida intermedia. Phytopathology 101: 859-869.

Karimi K, Babai Ahari A, Arzanlou M, Amini J, Pertot I, Rota-Stabelli O (2017) Application of the consolidated species concept to identify the causal agent of strawberry anthracnose in Iran and initial molecular dating of the Colletotrichum acutatum species complex. European Journal of Plant Pathology 147(2): 375-387. 

LaMondia JA (1995) Inhibition with benomyl to growth in vitro of Colletotrichum acutatum and C. fragaria and Strawberry fruit infection by benomyl-resistant isolates of C. acutatum. Advance strawberry Research 14: 25-30.

Larena I, Sabuquillo P, Melgarejo P, De Cal A (2003) Biocontrol of Fusarium and Verticillium wilt of tomato by Penicillium oxalicum under greenhouse and field conditions. Journal of Phytopathology 151: 507-512.

Larran S, Perello A, Simon MR (2007) The endophytic fungi from wheat (Triticum aestivum L.). World Journal of
Microbiology and Biotechnology 4: 565-572.

Larran S, Simon MR, Moreno MV, Santamarina Siurana MP, Perello A (2016) Endophytes from wheat as biocontrol agents against tan spot disease. Biological Control 92: 17-23.

Little ETM, Hills FG (1978) Agricultural experimentation: Design and analysis. John Willey and Sons. Inc. New York. USA. 349 pp.

Mass JL, (Ed.) (1998) Compendium of strawberry diseases. 2nd ed. APS Press, St. Paul, MN.

Mertely JC, Legard DE (2004) Detection, isolation, and pathogenicity of Colletotrichum spp. from strawberry petioles. Plant Disease 88: 407-416.

Moreira RR, Nesi VN, May De Mio LL (2014) Bacillus spp. and Pseudomonas putida as inhibitors of the Colletotrichum acutatum group and potential to control Glomerella leaf spot. Biological Control 72: 30-37.

Peres NA, Timmer LW, Adaskaveg JE, Correll JC (2005) Lifestyle of Colletotrichum acutatum. Plant Disease 89: 784-796.

Petrini O (1991) Fungal endophytes of tree leaves. In: Microbial Ecology of leaves. Springer-Verlag, New York, 497 pp.

Qualhato TF, Lopes FAC, Steindorff AC, Brandao RS, Jesuino RSA, Ulhoa CJ (2013) Mycoparasitism studies of Trichoderma species against three phytopathogenic fungi: evaluation of antagonism and hydrolytic enzyme production. Biotechnology Letter 35: 1461-1468.

Raeder U, Broda P (1985) Rapid preparation of DNA from filamentous Fungi. Letters in Applied Microbiology 1: 17-20.

Rosenblueth M, Martinez-Romero E (2006) Bacterial endophytes and their interaction with hosts. Molecular Plant Microbe Interaction 19: 827-837.

Sabuquillo P, De Cal A, Melgarejo P (2006) Biocontrol of tomato wilt by Penicillium oxalicum formulation in different crop conditions. Biological Control 37: 256-265.

Smith BJ, Black LL (1992) In vitro activity of fungicides against Colletotrichum fragariae.Acta Horticulturae 348: 509-512.

.Stadnik MJ, Araujo L, Valdebenito-Sanhueza RM (2009) Estrategias alternatives de controle da mancha foliar de glomerella e da saran da macieira. In: Stadnik. M.J. (Ed.). Manejo integrado de Doencas da Macieira. CCA-UFSC, Florianopolis. P. 105-126.

Strobet GA, Torzynski R, Bollon A (1997) Acremonium sp. a leucinostain, a producing endophyte of European yew (Taxus bacata). Plant Science 128: 97-106.

Swarthout D, Harper E, Judd S, Bultman T (2009) Measures of leaf-level water-use efficiency in drought stressed endophytes infected and non-infected tall fescue grasses. Environmental and Experimental Botany 66: 88–93.

Swofford DL (2003) Phylogenetic analysis using parsimony (and other methods). Version 4 Sunderland, Massachusetts Sinauer Associates.

Ting ASY, Chai JY (2015) Chitinase and ß-1, 3-glucanase activities of Trichoderma harzianum in response towards pathogenic and non-pathogenic isolates: early indications of compatibility in consortium. Biocatalysis and agricultural
biotechnology 4: 109-113.

Vega FE, Posada F, Aime MC, Pava-Ripoll M, Infante F, Rehner A (2008) Entomopathogenic fungal endophytes. Biological Control 46: 72-82.

Wedge DE, Smith BJ, Quebedeaux JP, Constantin RJ (2007) Fungicide management strategies for control of strawberry fruit rot diseases in Louisiana and Mississippi. Crop Protection 26: 1449-1458.