نوع مقاله : مقاله پژوهشی


1 ، پژوهشکده کشاورزی، دانشگاه زابل، ایران،

2 استادیار، گروه کشاورزی، دانشگاه آزاد اسلامی واحد زاهدان، زاهدان، پست الکترونیک Amehraban2004@yahoo.com :


این تحقیق با هدف بررسی اثر Bacillus methylotrophicus در کنترل قارچ Fusarium oxysporum f.sp. melonis و بیان برخی ژن‌های دفاعی خربزه انجام شد. این آزمایش به دو روش آغشته‌سازی خاک باB. methylotrophicus و آغشته‌سازی بذر با آن در شرایط گلخانه انجام شد. همچنین بیان ژن‌های (GAL2) galactinol synthase 2،β-1,3- glucanas 2 (βGlu2) ، (CAT) catalase و (Chi) chitinase با تکنیک Real-time PCR ارزیابی شد. گیاهان تیمار شده با B. methylotrophicus از نظر شدت بیماری اختلاف معنی‌داری با شاهد داشتند. شدت بیماری در روش آغشته‌سازی خاک با B. methylotrophicus نسبت به روش آغشته‌سازی بذر به-طور معنی‌داری کمتر بود. میزان بیان ژن‌هایGAL2 ، βGlu2، CAT و Chi به‌طور معنی‌داری توسطB. methylotrophicus افزایش یافت. نتایج این تحقیق نشان داد که B. methylotrophicus در کنترل بیماری پژمردگی ناشی از قارچF. o. f.sp. melonis و افزایش بیان ژن‌های دفاعی در گیاه خربزه موثر است. همچنین نتایج ما پیشنهاد می‌کند که B. methylotrophicus احتمالاً از طریق القا ژن‌های دفاعی باعث افزایش مقاومت خربزه در مقابلF. o. f.sp. melonis می‌شود.



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