76043abf2ebed2c

ارزیابی توان استرپتومیست‏های ریزوسفر گوجه‏فرنگی در کنترل قارچ بیمارگر Fusarium oxysporum f.sp. radicis-lycopersici

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه بیماری‌شناسی گیاهی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 دانشیار، گروه بیماری‌شناسی گیاهی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

پوسیدگی ریشه و طوقه گوجه‏فرنگی با عامل Fusarium oxysporum f.sp. radicis-lycopersici (FORL) یکی از بیماری‌های مهم خاکزاد گوجه‏فرنگی به‏حساب می‏آید. در این پژوهش 740 جدایه اکتینوباکتری‏‏ از مزارع گوجه‏فرنگی مناطق مختلف استان­های فارس و البرز جمع­آوری گردید. آزمون‏های آنزیمی انجام گرفته حاکی از تولید آنزیم‌ پروتئاز و توانایی تولید سیدروفور در تمامی جدایه‌ها بود. محیط کشت فیلتر شده جدایه UTS49 به‏عنوان جدایه برتر با جمعیت 108 × 3 به میزان 94 درصد از جوانه‌زنی اسپور قارچ بیمارگر جلوگیری کرد. بیشترین میزان تشکیل بیوفیلم باکتریایی مربوط به جدایه‏های UTS385، UTS10 و UTS49 بود. در بررسی‌های گلخانه‌ایجدایه UTS49 موجب کاهش علائم بیماری به میزان 75 درصد شد. بررسی جمعیت جدایه UTS49 در ریزوسفر خیار و گوجه‏فرنگی، بیانگر توان این جدایهدر کلنیزاسیون قوی و پایدار است. بر اساس آزمون‌های فوق جدایه UTS49به‏عنوان بهترین جدایه در کنترل بیماری پوسیدگی ریشه و طوقه گوجه‏فرنگی مشخص شد. با توجه به آزمونهای بیوشیمیایی و توالی rDNA 16s، این جدایه Streptomyces carpaticusشناسایی و معرفی گردید.

کلیدواژه‌ها

موضوعات


Alexander DB, Zuberer DA (1991) Use of chrome azurols reagents to evaluate siderophore production by rhizosphere bacteria.Biology and Fertility of Soils 12 (1): 39-45.

Arya R, Sharma KA (2016) Bioremediation of carbendazim, a benzimidazole fungicide using Brevibacillus borstelensis and Streptomyces albogriseolus together.Current Pharmaceutical Biotechnology 17 (2): 185-189.

Bajaj BK, Sharma P (2011) An alkali-thermotolerant extracellular protease from a newly isolated Streptomyces sp. DP2. New Biotechnology28 (6): 725-732.

Deepika L, Kannabiran K. (2009) Isolation and characterization of antagonistic Actinomyces from marine soil. Journal of Microbial and Biochemical Technology 65 (33): 19-29.

Doumbou CL, Hamby SMK, Crawford DL, Beaulieu C (2001) Actinomyces, promising tools to control plant diseases and to promote plant growth.Phytoprotection 82 (3): 85-102.

El-Abyad MS, El-Sayed MA, El-Shanshoury AR, El-Sabbagh SM (1993) Towards the biological control of fungal and bacterial diseases of tomato using antagonistic Streptomyces spp. Plant and Soil 149 (2): 185-195.

Enomoto Y, Shiomi K, Matsumoto A, Takahashi Y, Iwai Y, Harder A, Omura S (2001) Isolation of a new antibiotic oligomycin G produced by Streptomyces sp. WK-6150. The Journal of Antibiotics 54 (3): 308-313.

Getha K, Vikineswary S (2002) Antagonistic effects of Streptomyces violaceusniger strain G10 on Fusarium oxysporum f. sp. Cubense race 4: indirect evidence for the role of antibiosis in the antagonistic process. Journal of Industrial Microbiology and Biotechnology 28 (6): 303-310.

Gohel V, Singh A, Vimal M, Ashwini P, Chhatpar HS (2006) Review-bioprospecting and antifungal potential of chitinolytic microorganisms.Annual Review of Phytophatology 9 (1): 66-79.

Gonzalez-Franco CA, Robles-Hernandez RY (2009) Actinomyces as biological control agents of phytopathogenic fungi. Tecnociencia Chihuahua 3 (2): 64-73.

Gopalakrishnan S, Srinivas V, Vidya MS, Rathore A (2013) Plant growth-promoting activities of Streptomyces spp. In sorghum and rice.Springer plus 2 (1): 12.

Gopalakrishnan S, Vadlamudi S, Bandikinda P, Sathya A, Vijayabharathi R, Rupela O, Varshney RK (2014) Evaluation of Streptomyces strains isolated from herbal vermicompost for their plant growth-promotion traits in rice. Microbiological Research 169 (1): 40-48.

Hartman JR, Fletcher JT (1991) Fusarium crown and root rot of tomatoes in the UK. Plant Pathology 40 (1): 85-92.

Hibar K, Daami-Remadi M, Khiareddine H, El Mahjoub M (2005) Effet inhibiteur in vitro et in vivo du Trichoderma harzianum Sur Fusarium oxysporum f. sp. radicis-lycopersici. Biotechnologie Agronomie, Société et Environnement 9 (3): 163-171.

Hin-A-Woeng TF, Bloemberg GV, van der Bij AJ, van der Drift KM, Schripsema J, Kroon B, De Bruijn FJ (1998) Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici. Molecular Plant-Microbe Interactions11 (11): 1069-1077.

Hsu SC, Lockwood JL (1975) Powdered chitin agar as a selective medium for enumeration of Actinomyces in water and soil. Applied Microbiology 29 (3): 422-426.

Hu HQ, Li XS, He H (2010) Characterization of an antimicrobial material from a newly isolated Bacillus amyloliquefaciens from mangrove for biocontrol of Capsicum bacterial wilt. Biological Control 54 (3): 359-365.

Hu L, Wang D, Liu L, Chen J, Xue Y, Shi Z (2013) Ca 2+ efflux is involved in cinnamaldehyde-induced growth inhibition of Phytophthora capsici. Plos one 8 (10): 76-0264.

Igo N (1983) Survey of greenhouse management practices in Essex County, Ontario, in relation to Fusarium foot and root rot of tomato. Plant Disease 67 (1): 36-79.

Jarvis WR, Shoemaker RA (1978) Taxonomic status of Fusarium oxysporum causing foot and root rot of tomato. Phytopathology 39 (2): 213-222.

Kanini GS, Katsifas EA, Savvides AL, Karagouni AD (2013) Streptomyces rochei ACTA1551, an indigenous Greek isolate studied as a potential biocontrol agent against Fusarium oxysporum f. sp. lycopersici. Biomedical Research International 2013(1): 56-75.

Kim JT, Park IH, Hahm YI, Yu SH (2001) Crown and root rot of greenhouse tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici in Korea. The Plant Pathology Journal 17 (5): 290-294.

Lu D, Ma Z, Xu X, Yu X (2016) Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f. sp. cucumerinum. Journal of Basic Microbiology 60 (2): 271-280.

Martinez-Viveros O, Jorquera MA, Crowley DE, Gajardo GMLM, Mora ML (2010) Mechanisms and practical considerations involved in plant growth promotion by rhizobacteria. Journal of Soil science and Plant Nutrition 10 (3): 293-319.

Minuto A, Spadaro D, Garibaldi A, Gullino ML (2006) Control of soilborne pathogens of tomato using a commercial formulation of Streptomyces griseoviridis and solarization. CropProtection 25 (5), 468-475.

Nei M, Kumar S (2000). Molecular evolution and phylogenetics. Oxford University. Press New York United State.

Nirmaladevi D, Venkataramana M, Srivastava RK, Uppalapati SR, Gupta VK, Yli-Mattila T, Chandra NS (2016) Molecular phylogeny, pathogenicity and toxigenicity of Fusarium oxysporum f. sp. lycopersici. Scientific Reports 6 (1): 423-436.

Nolan RD, Cross TS (1988) Isolation and screening of Actinomyces. Actinomyces in Biotechnology 1-32.

Oliveira MD, Varanda CMR, Félix MRF (2016) Induced resistance during the interaction pathogen x plant and the use of resistance inducers. Phytochemistry Letters 15 (1): 152-158.

Quecine MC, Araujo WL, Marcon J, Gai CS., Azevedo JL, Pizzirani-Kleiner AA (2008) Chitinolytic activity of endophytic Streptomyces and potential for biocontrol.Letters in Applied Microbiology 47 (6): 486-491.

Rowe RC, Farley JD (1981) Strategies for controlling Fusarium crown and root rot in greenhouse tomatoes. Plant Disease 65 (2): 107-112.

Sabaratnam S, Traquair JA (2002) Formulation of a Streptomyces biocontrol agent for the suppression of Rhizoctonia damping-off in tomato transplants. Biological Control 23 (3): 245-253.

Saha M, Sarkar S, Sarkar B, Sharma BK, Bhattacharjee S, Tribedi P (2016) Microbial siderophores and their potential applications: a review. Environmental Science and Pollution Research 23 (5): 3984-3999.

Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4 (4): 406-425.

Schaad NW (2001) Initial identification of common genera. Laboratory guide for identification of plant pathogenic bacteria. APS Press. 373 pp.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution30(12): 2725-2729.

Tokala RK, Strap JL, Jung CM, Crawford DL, Salove MH, Deobald LA, Morra MJ (2002) Novel plant-microbe rhizosphere interaction involving Streptomyces lydicus WYEC108 and the pea plant (Pisum sativum). Applied and Environmental Microbiology 68 (5): 2161-2171.

Weller DM (1988) Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annual Review of Phytopathology 26 (1): 379-407.

Williams ST, Goodfellow M, Alderson G, Wellington EMH, Sneath PHA, Sackin MJ (1983) Numerical classification of Streptomyces and related genera. Microbiology 129 (6): 1743-1813.

Winn M, Casey E, Habimana O, Murphy CD (2014) Characteristics of Streptomyces griseus biofilms in continuous flow tubular reactors. FEMS Microbiology Letters 352 (2): 157-164.

Yamamoto I, Komada H, kyniyasu K, Saito M, Ezuka A (1974) A new race of Fusarium oxysporum f. sp. lycopersici inducing root rot of tomato. Plant Protection Society 81 (1): 284-287.

Yan Z, Reddy MS, Kloepper JW (2003) Survival and colonization of Rhizobacteria in a tomato transplant system. Canadian Journal of Microbiology 49 (6): 383-389.

Yuan WM, Crawford DL (1995) Characterization of Streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. Applied and Environmental Microbiology 61 (8): 3119-3128.

Zhao S, Du CM, Tian CY (2012) Suppression of Fusarium oxysporum and induced resistance of plants involved in the biocontrol of Cucumber Fusarium Wilt by Streptomyces bikiniensis HD-087. World Journal of Microbiology and Biotechnology 28 (9): 2919-2927.