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تأثیر بیوفیلم تولید شده توسط ریزوباکتری‌های پروبیوتیک گیاهی بر کلنیزاسیون ریشه و رشد گیاه گندم

نوع مقاله : مقاله پژوهشی

نویسنده

استادیار گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه ارومیه

چکیده

به منظور بررسی پتانسیل باکتری پروبیوتیک Bacillus subtilis در تولید بیوفیلم و کلنیزاسیون ریشه گندم، از 11 سویه این باکتری استفاده شد. آزمایش­ها در قالب طرح کاملا تصادفی و در چهار تکرار انجام شد. بنابر نتایج حاصل از این پژوهش، میزان تولید بیوفیلم و بیوسورفکتانت­ها در ریزوباکتری­های مورد مطالعه تنوع بالایی نشان داد و بیشترین مقدار تولید این متابولیت­ها در سویه­های B3، B1 و B4 دیده شد. بررسی میزان کلنیزاسیون با استفاده از باکتری­های موتانت مقاوم به آنتی­بیوتیک­های ریفامپی­سین و نالیدیکسیک­اسید انجام شد. سویه­های B4 و B3  به ترتیب با جمعیت­های FU/g  107×41/4 و cfu/g 107×35/4، بیشترین میزان کلنیزاسیون ریشه را نسبت به سایر سویه­ها داشتند. میزان تشکیل بیوفیلم و تولید بیوسورفکتانت­ها با کلنیزاسیون ریشه گندم به ترتیب 74/0 و 80/0 همبستگی داشتند. در بخش دیگر این تحقیق، اثر این سویه­ها روی فاکتورهای رشدی گیاه گندم مورد ارزیابی قرار گرفت. کلیه سویه­ها موجب افزایش طول ریشه و اندام هوایی شدند و بیشتر سویه­ها اثر افزایشی در وزن خشک ریشه و اندام هوایی نشان دادند. براساس یافته­های این پژوهش، سویه­های باکتری که پتانسیل بالایی در تولید بیوفیلم و بیوسورفکتانت­ها داشتند، ریشه گندم را به خوبی کلنیزه نمودند و غالب سویه­ها اثر افزاینده روی فاکتورهای رشدی گندم نشان دادند.

کلیدواژه‌ها

موضوعات


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