76043abf2ebed2c

بررسی قابلیت مهارکنندگی تعدادی از سویه‌های Pseudomonas fluorescens روی بیماری بوته‌میری خیار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه گیاه‌پزشکی، دانشکدة کشاورزی، دانشگاه ولی‌عصر (عج)، رفسنجان

2 استادیار، گروه گیاه‌پزشکی، دانشکدة کشاورزی، دانشگاه ولی‌عصر (عج)، رفسنجان

چکیده

در این مطالعه، اثر بیوکنترلی برخی سودومونادهای فلورسنت در برابر Pythium aphanidermatum عامل بیماریبوته‌میری خیاربررسیشد. در ابتدا، توانایی آنتاگونیستی سویه‌های باکتریایی علیه قارچ بیمارگر با استفاده از روش کشت متقابل ارزیابی شد. نتایج نشان داد که هالة بازدارندگی سویة T17-4 (5/7 میلی‌متر) بیشتر از سایر سویه‌ها بود. هفت سویه شامل سویة T17-4، CHA0، CHA89، VUPf52، VUPf506، VUPf760 و VUPf5 که بهترین اثر را روی کنترل چندین بیماری داشته‌اند برای مرحلة گلخانه انتخاب شدند. همچنین، سویه‌های مذکور پارامترهای رشدی گیاه شامل ارتفاع و وزن تر و خشک اندام هوایی و ریشه را افزایش دادند. نتایج تغییرات دفاعی نشان داد که بیشترین میزان افزایش فعالیت آنزیم پراکسیداز و میزان فنل کل و پلی‌فنل‌اکسیداز، نه روز پس از مایه‌زنی قارچ عامل بیماری، مشاهده شدند و سپس، کاهش یافتند. میزان افزایش در فعالیت آنزیم پراکسیداز و میزان فنل کل و پلی‌فنل‌اکسیداز در تیمارهای حاوی باکتری و قارچ به‌صورت توأم، در مقایسه با تیمار شاهد معنی‌دار بود.

کلیدواژه‌ها

موضوعات


Akram W, Anjum T, Ali B, Ahmad A (2013-) Screening of native bacillus strains to induce systemic resistance in tomato plants against Fusarium wilt in split root system and its field applications. International Journal of Agriculture and Biology 15: 1289-1294.
Alabouvette C, Olivain C, Steinberg  C (2006) Biological control of plant diseases: the European situation. European journal of plant pathology 114: 329-341.
Bakker AW, Schippers B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas sp. mediated plant growth stimulation. Soil Biology and Biochemistry 19(4): 451-457.
Bradford  MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 72: 248-254.
Chen C, Belanger RR, Benhamou N, Paulitz TC (2000) Defense enzymes induced in cucumber roots by treatment with plant growth-promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiological and Molecular Plant Pathology 56: 13-23.
Cummings SP (2009) The application of plant growth promoting rhizobacteria (PGPR) in low input and organic cultivation of graminaceous crops; potential and problems. Environmental Biotechnology 5(2):43-50.
Eifediyi EK, Remison SU (2010) Growth and yield of cucumber (Cucumis sativus L.) as influenced by farmyard manure and inorganic fertilizer. Journal of Plant Breeding and Crop Science 2: 216-220
El-Khallal SM (2007) Induction and modulation of resistance in tomato plants against Fusarium wilt disease by bioagent fungi (arbuscular mycorrhiza) and/or hormonal elicitors (jasmonic acid and salicylic acid): 2-changes in the antioxidant enzymes, phenolic compounds and pathogen related-proteins. Australian Journal of Basic and Applied Sciences 1: 717-732.
Fravel DR (1988) Role of antibiosis in the biocontrol of plant disease. Annual Review of Phytopathology 26: 75-91.
Fric F (1976) Oxidative enzymes, In: Heitefuss R, Williams PH (eds.), Encyclopedia of plant physiology. Springer, Berlin Hidelberg New York, 4: 617 - 631.
Fujita S, Saari NB, Maegawa M, Tetsuka T, Hayashi N, Tono T (1995) Purification and properties of polyphenol oxidase from cabbage (Brassica oleracea L.). Journal of Agricultural and Food Chemistry 43: 1138-1142.
Goodman RN, Király Z, Wood KR (1986) The biochemistry and physiology of plant disease. University of Missouri Press.
Jung W J, Mabood F, Kim TH, Smith DL (2007) Induction of pathogenesis-related proteins during biocontrol of Rhizoctonia solani with Pseudomonas aureofaciens in soybean (Glycine max L. Merr.). Biological Control of Plant Pathogens 52: 895-904.
Hagedorn C, Gould WD, Bardinelli TR (1989) Rhizobacteria of cotton and their repression of seedling disease pathogens. Applied and Environmental Microbiology 55: 2793-2797.
Harish S, Kavino M, Kumar N, Saravana Kumar D, Soorianathasundaram K, Samiyappan R (2008) Bio hardening with plant growth promoting rhizosphere and endophytic bacteria induces systemic resistance against banana bunchy top virus. Applied Soil Ecology 39: 187-200.
Keel C, Défago G (1997) Interactions between beneficial soil bacteria and root pathogens: mechanisms and ecological impact. Multitrophic interactions in terrestrial systems: 27-46.
Keel C, Weller DM, Natsch A, Défago G, Cook RJ, Thomashow LS (1996) Conservation of the 2, 4-diacetylphloroglucinol biosynthesis locus among fluorescent Pseudomonas strains from diverse geographic locations. Applied and Environmental Microbiology 62: 552-563.
Khatamidoost Shekarsaraee Z (2014) Effect of Pseudomonas fluorescens in control of pistachio root knot nematode in green house conditions. M.Sc., University of Guilan, Guilan, Iran. (in Persian)
Li L, Steffens JC (2002) Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta 215: 239-247.
MacAdam  JW, Nelson CJ, Sharp RE (1992) Peroxidase activity in the leaf elongation zone of tall fescue I. Spatial distribution of ionically bound peroxidase activity in genotypes differing in length of the elongation zone. Plant Physiology 99: 872-878.
Matern U, Kneusel RE (1988) Phenolic compounds in plant disease resistance. Phytoparasitica 16: 153-170.
Murthy KN, Uzma F, Srinivas CC (2014) Induction of systemic resistance in tomato against Ralstonia solanacearum by Pseudomonas fluorescens. American Journal of Plant Sciences 5: 1799.
Nandakumar R, Babu S, Viswanathan R, Raguchander T, Samiyappan R (2001) Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens. Soil Biology & Biochemistry 33: 603-612.
Odjakova M, Hadjiivanova C (2001) The complexity of pathogen defense in plants. Bulgarian Journal of Plant Physiology 27: 101-109.
Ownley BH, Duffy BK, Weller DM (2003) Identification and manipulation of soil properties to improve the biological control performance of phenazine-producing Pseudomonas fluorescens. Applied and Environmental Microbiology 69: 3333-3343.
Ozgonen H, Erkilic A (2007) Growth enhancement and Phytophthora blight (Phytophthora capsici Leonian) control by arbuscular mycorrhizal fungal inoculation in pepper. Crop Protection 26: 1682-1688.
Raj SN, Sarosh BR, Shetty HS (2006) Induction and accumulation of polyphenol oxidase activities as implicated in development of resistance against pearl millet downy mildew disease. Functional Plant Biology 33: 563-571.
Rostami F, Alaei H, Karimi, HR (2010) Control off cucumber daming off (Phytophthora drechsleri Tucker) using cucumber transplan on pumpkin. Proceedings of the 19th Iranian Plant Protection Congress, 31 July- 3 August, Tehran, Iran. (in Persian)
Picard C, Cello I, Ventura  M, Fani R, Guckert A (2000) Frequency and biodiversity of 2,4-diacetyphloroglucinol production bacteria isolated from the maize rhizosphere at different stages of plant growth. Applied and Environmental Microbiology 66: 948-955.
Raupach, GS, Kloepper  JW, (1998) Mixtures of plant growth- promoting rhizobacteria enhance biological control of multiple excumber pathogens. Journal of Phytopatholoy 88: 1158- 1164.
Reuveni, R (1995) Biochemical marker for disease resistance, In: Singh RP, Singh US (eds), Molecular methods in plant pathology. CRC Press, Boca Raton, FL: 99-144.
Salman M, Abuamsha R, Barghouthi, S (2013) Interaction of fluorescent pseudomonads with Pythium ultimum and Rhizoctonia solani in cucumber roots. American Journal of Experimental Agriculture 3(1): 240-251.
Schippers B, Bakker AW, Bakker PAHM (1987) Interactions of deleterious and beneficial rhizosphere microorganisms and the effect of cropping practices. Annual Review of Phytopathology 25: 339-358.
Soland SF, Laima SK (1999) Phenolics and cold tolerance of Brassica napus. Plant Agric 1: 1-5.
Spadaro D, Gullino ML (2005) Improving the efficacy of biocontrol agents against soilborne pathogens. Crop Protection 24: 601-613.
Van Loon LC (2007) Plant responses to plant growth-promoting rhizobacteria. European Journal of Plant Pathology 119: 243-254.
Van Loon LC, Van Strien EA (1999) The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiological and Molecular Plant Pathology, 55: 85-97.
Weller DM (1988) Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annual Review of Phytopathology 26: 379-407.
Yamamoto H (1995) Pathogenesis and host-parasite specificity in rusts, In: Kohmoto K, Singh V, Singh RP (eds.), Plant disease histopathological. Biochemical, genetic and molecular bases. Vol. ΙΙ. Eukaryots. Pergam.