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بررسی زهرآگینی و نحوه تندش کنیدی جدایه های مختلف قارچ Beauveria bassiana

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی دکترا-دانشگاه ارومیه

2 دانشگاه ارومیه

3 گروه گیاهپزشکی - دانشگاه تهران

4 دانشگاه تهران

چکیده

در پژوهش حاضر، جداسازی جدایه‌های مختلف قارچ Beauveria bassiana از خاک مناطق مختلف و بررسی زهرآگینی آن‌ها علیه سن گندم(Hem.: Scutelleridae) Eurygaster integriceps، سوسک Zophobas morio (Col.: Tenebrionidae) و شب‌پره آرد (Lep.: Pyralidae) Ephestia kuehniella انجام شد. از نمونه‌های خاک مناطق کرج، ارومیه، ماکو و دزفول، 23 جدایه قارچ B. bassiana توسط تله گالریا، جداسازی و شناسایی شد. حشرات به روش غوطه‌وری با این جدایه‌ها آلوده شدند، بر اساس نتایج پوره‌های سن 5 سن گندم و لاروهای سن 4 شب‌پره آرد به تمام جدایه‌ها حساس بودند درحالی که لارو سن آخر سوسک مقاوم بود و هیچ مرگی ثبت نشد. بیش‌ترین تلفات به‌وسیله‌ی جدایه‌های TV و TZ با حدود 90 درصد مرگ روی هر دو میزبان E .kuehniella و E. integriceps مشاهده شد که در بالاترین گروه زهرآگینی قرار گرفتند. بر اساس زهرآگینی، جدایه‌ها به سه دسته تقسیم‌بندی شدند: جدایه‌های با زهر‌آگینی کم (ایجاد 10 تا 33 درصد مرگ)، جدایه‌های با زهر‌آگینی متوسط (ایجاد 34 تا 67 درصد مرگ) و جدایه‌های با زهر‌آگینی زیاد (ایجاد 68 تا 100 درصد مرگ). روی محیط کشت PDA دو نوع تندش یک‌طرفه و دوطرفه برای کنیدی‌های جدایه‌ها تشخیص داده شد. درصد این دو نوع تندش بین جدایه‌ها تفاوت معنی‌داری داشت، بین نوع تندش و زهرآگینی همبستگی معنی‌داری دیده نشد.

کلیدواژه‌ها

موضوعات


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