76043abf2ebed2c

بهینه‌سازی محیط کشتBG11 به‌منظور افزایش تأثیر ضدقارچی سیانوباکتری Cyanobacterium sp. PGU1 علیه برخی بیمارگرهای قارچی گیاهان

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد بیوتکنولوژی دریا، دانشکدۀ علوم و فنون دریایی، دانشگاه خلیج‌فارس (بوشهر)

2 استادیار، گروه اصلاح نباتات، دانشکدۀ کشاورزی و منابع طبیعی دانشگاه خلیج‌فارس، بوشهر

3 استادیار، مرکز آموزش عالی جهاد کشاورزی میرزاکوچک خان (گیلان)

چکیده

سیانوباکتری­ها به­علت تولید انواع متابولیت­های ثانویه و ترکیبات ضد­میکروبی موردتوجه بسیاری از محققان قرارگرفته‌اند. این تحقیق به‌منظور بهینه­سازی محیط کشت BG11 با هدف افزایش تأثیر ضد­قارچی سیانوباکتری Cyanobacteriumsp.PGU1 علیه دو قارچ بیماریزای گیاهی Fusarium oxysporum f. sp. lycopersici و Rhizoctonia solani AG2-2-IIIB انجام شد. سیانوباکتری جداسازی‌شده از آب خلیج‌فارس با دو روش ریخت‌شناختی (مورفولوژیک) و تعیین توالی 16S rRNA با عنوان Cyanobacterium sp. PGU1 شناسایی شد. بهینه­سازی محیط کشتBG11، با تغییر میزان منابع نیتروژن و فسفات موجود در محیط کشت، هرکدام در سه سطح، شامل 75/0، 5/1 و 3 گرم در لیتر برای NaNO3 و میزان 02/0، 04/0 و 08/0 گرم در لیتر برای  K2HPO4انجام شد و تأثیر این مقادیر بر فعالیت ضد قارچی سیانوباکتری بررسی شد. تأثیر ضد­قارچی عصارۀ متانولی به‌دست‌آمده از کشت خالص سیانوباکتری، پیش و پس از بهینه‌سازی به روش اختلاط عصاره در محیط کشت بررسی شد. پس از بهینه­سازی محیط کشت، درصد جلوگیری از رشد F. oxysporum f. sp. lycopersici از 33/79 به 43/87 درصد و برای AG2-2-IIIB  R. solani از 47/82 به 62/90 درصد افزایش یافت.

کلیدواژه‌ها

موضوعات


Abdelillah A, Houcine B, Halima D, Meriel C, Imane Z, Eddine SD, Abdallah M, Daoudi CS (2013) Evaluation of antifungal activity of free fatty acids methyl esters fraction isolated from Algerian  Inumusita tissimum L. seeds against toxigenic Aspergillus. Journal of Tropical Biomedicine 3(6): 443-448.

Abo-Shady AM, Ghaffar BA, Rahhal MMH, Monem HA ( 2007) Biological control of faba bean pathogenic fungi by three cyanobacterial filtrates. Pakistan Journal of Biological Sciences 10(18): 3029-3038.

Abu-Nada Y (2006) Metabolic profiling of potato cultivars varying in horizontal resistance to late blight, Phytophthora infestans. Ph.D. thesis, McGill University, Canada.

Agoramoorthy G, Chandrasekaran M, Venkatesalu V, Hsu MJ (2007) Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from India. Brazilian Journal of Microbiology 38: 739-742.

Alwathnani HA, Perveen K (2012) Biological control of Fusarium wilt of tomato by antagonist fungi and cyanobacteria. African Journal of Biotechnology 11(5): 1100-1105.

Anand NL, Radha RS, Ravati G, Subramianam TD (1990) Perspectives in phycology. Today and Tomorrow's Printers and Publishers New Delhi. pp. 383-391.

Azarm A, Mohajer R (2010) The role of cyanobacteria biological fertilizer in improving the quality of soil. Proceeding of the first Congress of Fertilizer Challenges in Iran: half century of application of fertilizer, 2010, Tehran, Iran. (in Persian)

Basha M, Bhatnagar A (2010) Biodiversity of microalgae and cyanobacteria from fresh water bodies of Jodhpur, rajasthan (India). Journal of Algal Biomass 1(3): 45-69.

Cardoso VM, Solano AG, Prado MA, Nunan A (2006) Investigation of fatty acid esters to replace isopropyl myristate in the sterility test for ophthalmic ointments. Journal of Pharmaceutical and Biomedical Analysis  42(5): 630-4.

Fiore M, Neilan BA, Copp JN, Rodrigues JLM, Tsai M, Lee H, Trevors JT (2005). Characterization of nitrogen-fixing cyanobacteria in the Brazilian Amazon floodplain.Water research 39: 5017-5026.

Goldberg IK, Cohen Z (2006) The effect of phosphate starvation on the lipid and fatty acid composition of the fresh water eustigmatophyte Monodus subterraneus. Phytochemistry 67: 696-701.

Graham GC, Mayer P, Henry RJ (1994) A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotechniques 1: 48-50.

Heidari F, Riahi H, Yousefzadi M, Asadi M (2012) Antimicrobial activity of cyanobacteria isolated from hot spring of Geno. Middle-East Journal of Scientific Research 12(3): 336-339.

Huang CB, George B, Ebersole JL(2010) Antimicrobial activity of n-6, n-7 and n-9 fatty acids and their esters for oral microorganisms. Archives of Oral Biology 55(8): 555-560.

Khairy H, El-Kassas H (2010) Active substance from some blue green algal species used as antimicrobial agents. African Journal of Biotechnology 9(19): 2789-2800.

Kathiresan S, Sarada R, Bhattacharya SGA (2006) Culture media optimization for growth and phycoerythrin production from Porphyridium purpureum. Biotechnology and Bioengineering 96(3): 456-463.

Kulik MM (1995) The potential for using cyanobacteria (blue-green algae) and algae in the biological control of plant pathogenic bacteria and fungi. European Journal of Plant Pathology 101: 585-599.

Latysheva N, Palmer J, Codd GA, Barker D (2012) The evolution of nitrogen fixation in cyanobacteria. Bioinformatics 28(5): 603-606.

Lin J, Dou J, Xu J, Aisa HA (2012) Chemical composition, antimicrobial and antitumor activities of the essential oils and crude extracts of Euphorbia macrorrhiza. Molecules 17: 5030-5039.

Martineau E, Susanna A, Matthew R, Miller J, Anne D, Hawes JI, Webster-Brown J,  Packer MA (2013) Characterisation of antarctic cyanobacteria and comparison with New zealand strains. Hydrobiologia 711:139-154.

Mikaeili A, Karimi T, Shamspur T, Gholamine B, Modaresi M, Khanlari M (2012) Anti-candidal activity of Astragalus verus in the in vitro and in vivo guinea pig models of cutaneous and systemic candidiasis. Brazilian Journal of Pharmacognosy 22(5): 1035-1043.

Mohammadi F, Heshmatipour Z, Khanafari A, Njafi S, Hajipour S (2012) Isolation and identification of thermophilic cyanobacteria from  hot spring in Ramsar. The 13th Iranian and the 2nd international congress of Microbiology, Ardabil, Iran, 121.

Morin N, Vallaeys T, Hendrickx L, Natalie L, Wilmotte N (2010) An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira. Journal of Microbiological Methods 80 (2): 148–154.

Prescott, GW (1982) Algae of the western great lakes areas William, C. Brown Company publisher, Dubuqe, Iowa.

Pohl CH, Kock JLF, Thibane VS (2011) Antifungal free fatty acids: a Review In: Mendes-yalis A (ed.), Science against microbial pathogens: communicating current research and technological advances. FORMATEX, France, Pp 61-71.

Rodrigues LA, Lima DS, Johann S, Cisalpino PS, Pinheiro L, Pimenta S, AméliaBoaventura M (2011) In vitro antifungal activity of fatty acid methyl esters of the seeds of Annona cornifolia A. St.-Hil. (Annonaceae) against pathogenic fungus Paracoccidioides brasiliensis. Revista da Sociedade Brasileira de Medicina Tropical 44(6): 777-780.

Saha SK, Uma L, Subramanian G (2003) Nitrogen stress induced changes in the marine cyanobacterium Oscillatoria willei BDU 130511. FEMS Microbiology Ecology 45: 263-272.

Salem O, Hoballah E, Ghazi S, Hanna S (2014) Antimicrobial activity of microalgal extracts with special emphasize on Nostoc sp.. Life Science Journal 11(12): 752-758.

Samosorn A, Pumnuan J, Insung A, Ruangsomboon S (2007) Effects of nitrate and phosphate in culture medium on production of bioactive compounds by Oscillatoria sp. affecting on the house dust mite, Rmatophagoides pteronyssinus (Trouessart).  The 8th International Symposium on Biocontrol and Biotechnology, 215-219.

Sharma V, Singh G, Kaur H, Saxena A K, Ishar MP (2012) Synthesis of β-ionone derived chalcones as potent antimicrobial agents. Journal of Bioorganic and Medical Chemistry Letters 22(20): 6343-6.

Soltani N, Dezfoulian, Shokravi Sh, Bafteh-Chi L, Ehsan Sh (2008) Isolation and morphological and molecular characterization of  new species of cyanobacteria from firooz-kooh area (Tehran province) using different media cultures. Science Journal 8(4): 319-328. (in Persian)

Takamatsu S, Nagle DG, Gerwick WH (2004) Secondary metabolites from marine cyanobacteria and algae inhibit LFA-1/ICAM-1 mediated cell adhesion. Journal of Natural Products 70(2):127-31.

Tantawy STA (2011) Biological potential of cyanobacterial metabolites against some soil pathogenic fungi. Journal of Food, Agriculture & Environment 9 (1): 663-666.

Takagi M, Watanabe K, Yamaberi K, Yoshida Y (2000) Limited feeding of potassium nitrate for intracellular lipid and triglyceride accumulation of Nannochloris sp. Applied Microbiology and Biotechnology 54, 112-117.

Tong Tan, L (2007) Bioactive natural products from marine cyanobacteria for drug discovery. Phytochemistry 68: 954-979.

Viyoch J, Pisutthanan N, Faikreua A, Nupangta K, Wangtorpol K, Ngokkuen J (2006) Evaluation of in vitro antimicrobial activity of Thai basil oils and their micro-emulsion formulas against Propioni bacterium acnes. International Journal of Cosmetic Science 28(2): 125-33.

Vonshak A (1986) Laboratory techniques for the cultivation ofrnicroalgae. In: Richmond A (ed.), Handbook of micro algal mass culture. CRC Press, Boca Raton, FL. pp. 89-95

Wada H, Murata M (1990) Temperature-induced changes in the fatty acid composition of the cyanobacterium, Synechocystis PCC68031.Plant Physiology 92: 1062-1069.

Xiaobo Z, haiying W, Linyu H, Yongcheng L, Zhongtao L (2006) Medium optimization of carbon and nitrogen sources for theproduction of eucalyptene A and xyloketal A from Xylaria sp. 2508 using response surface methodology.Process Biochemistry 41: 293-298.

Yoon MY, Choi GJ, Choi, YH, Jang KS, Cha B, Kim JC (2011) Antifungal activity of polyacetylenes isolated from Cirsium japonicum roots against various phyto pathogenic fungi. Journal of Industrial Crops and Products 34(1): 882-887. 

Zarrini G, Rasooli I, Abazari M, GhasemiY (2011). Investigation of antimicrobial activity of cyanobacteria isolated from urmialake catchment area. Journal of Ardabil University Medical Sciences 11(4): 329-336. (in Persain)

Zhang W, Feng Y (2008) Characterization of nitrogen-fixing moderate halophilic cyanobacteria isolated from saline soils of Songnen Plain in China. Progress in Natural Science 18: 769-773.