Evaluate the control ability of Pseudomonas fluorescens strains on cucumber root rot disease

Document Type : Complete paper

Authors

1 M. Sc. Student, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University, Rafsanjan, Iran

2 Assistance Professor, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University, Rafsanjan, Iran

Abstract

In this study, biocontrol effect of some strains Pseudomonas fluorescent surveyagainst Pythium aphanidermatum the causal agent of cucumber root rot disease. First biocontrol ability of bacteria against the fungal pathogen evaluated by cross-culture method. Results showed that inhibition zone of strain T17-4 (7.5 mm) was more than others. Seven strains including T17-4, CHA0, CHA89, VUPf52, VUPf506, VUPf760 and VUPf5 that had the best effect on control of some disease were selected for greenhouse conditions. The result of this experiment showed VUPf52, VUPf5 and T17-4 strains with 50, 49 and 43% (toward infected treatment) had the highest inhibition of cucumber root rot disease, respectively. These strains promote plant growth parameters such as height, dry and wet weight of shoot and root, too. The defense changes showed the highest increasing in peroxidase activity, total phenolic content and polyphenol oxidase after nine days inoculation and then decreased. The rate of increase in peroxidase activity, total phenolic content and polyphenol oxidase in treatments combined with bacteria and fungi compared with control treatment was significant.

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References

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BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 4, Issue 2 - Serial Number 2
Autumn & Winter
October 2015
Pages 77-88

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