Efficacy of Pseudomonas fluorescens strain EB298 (UTPf298) against Fusarium pseudograminearum crown and root rot disease of wheat and improving the efficiency of chlorophyll pigments

Document Type : Complete paper

Authors

1 Student of University of Tehran

2 Department of Biotecnology,Isfahan University of Technology

Abstract

Wheat is one of the most important agricultural products with the cultivated area covering about half of Iran's arable land. Fusarium pseudograminearum crown and root rot disease of wheat is one of the most destructive fungal agents in dry regions of Iran and the world, which affects the production of this product. By destroying the chlorophyll of the wheat plant, this disease reduces the growth of the plant and over time causes the death and complete drying of the wheat plant. The use of chemical poisons is one of the ways to control the damage caused by this disease, but due to the environmental damage caused by these poisons, scientists are looking for an alternative method. In this study, with the help of Pseudomonas fluorescens strain EB298 (UTPf298), the pathogenicity of this fungus in greenhouse conditions was significantly reduced compared to the control plant. In laboratory conditions, the bacterial isolate prevented the growth of the fungus by 70%. Also, this isolate has increased the amount of chlorophyll a, b and total chlorophyll to 1, 0.5 and 1.5 mg/g, respectively, by reducing the damage caused by the fungus. By stimulating plant growth, Pseudomonas fluorescens strain EB298 (UTPf298) increased the weight of root and stem by 0.35 and 0.9 grams and increased the length of root and stem by 15 and 28 cm. Therefore, in this research, due to the efficiency of Pseudomonas fluorescens EB298 (UTPf298), it can be used to control Fusarium pseudograminearum crown and root rot disease of wheat .

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References

سمیعی، ف.، جوان نیکخواه، م.، زمانی زاده، ح.ر. و رفیعی کوهرودی، ز. 1387. واکنش تعدادی از ارقام گندم به قارچ Bipolaris sorokiniana عامل پوسیدگی معمولی ریشه. نشریه حفاظت گیاهان (علوم و صنایع کشاورزی). 22: 211-219.
Anagholi, A., Tabatabaii, S. and Fouman Ajirlou, A. (2010). Evaluation of Salt-tolarance of varieties of Sorgom using sensitivity and stress-tolerance indices. Agricultural Crop Production. 3(1): 89-102.
Baghbani, F., Lotfi, R,. Moharramnejad, S., Bandehagh, A., Roostaei, M,. Rastogi, A. and Kalaji, H. (2019). Impact of Fusarium verticillioides on chlorophyll fluorescence parameters of two maize lines. European Journal of Plant Pathology. 154: 337-346.
Dehghan, A., Gregorian, A. and Hashemi, M. (2013). Determine the damage caused by fungal root rot diseases of wheat in the province. Iranian Journal of Applied Plant Protection. 3(1): 273-279
Egamberdieva, D., Jabborova, D. and Hashem, A. 2015. Pseudomonas induces salinity tolerance in cotton (Gossypium hirsutum) and resistance to Fusarium root rot through the modulation of indole-3-acetic acid. Saudi Journal of Biological Sciences. 22: 773-779.
Fradkin, L. G., Yoshinaga, S. K., Berk, A. J. and Dasgupta, A. (1987). Mol. Cell. Biology, 7: 3880_3887.
Gunes, A., Inal, A., Alpaslan, M., Eraslan, F., Bagci, E. and Cicek, N. (2007). Salcylic acid indiuced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize grown under salinity. Journal Plant Physiology, 164 (6): 728-36.
Iranian Agriculture News Agency -IANA. (2022). http://www.iana.ir.
Kazan, K. And Gardiner, D. M. (2018). Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects. Molecular Plant Pathology, 19 (7); 1547–1562.
Marasco, R., Rolli, E., Ettoumi, B., Vigani, G., Mapelli, F., Borin, S. and Dafonchio, D. (2012). A drought tesistance_promoting microbiome is selected by root system. PloS ONE. 7 (10): e48479.
Nadeem, S., Zahir, Z. A., Naveed, M. and Arshad, M. (2007). Preliminary inverstigations on inducing salt tolerance in Canola through ACC-deaminase activity. Canadian Journal of Microbiology. 53(10):1141-1149.
Ongena, M. and Jacques, Ph. (2008). Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends Microbiol. 16(3): 115-25.
PanelAbeer, H., Akhter,  A,.   Abdulaziz, A. A., Singh, G., Khalid. F. A. and Fathi, E. (2021).  Mycorrhizal fungi induced activation of tomato defense system mitigates Fusarium wilt stress. Saudi-journal-of-biological-sciences. 28 (10): 5442-5450.
Ravanbakhsh, M., Sasidharan, R,. Voesenek, L. A. C. J,. Kowalchuk, G. A. and Jousset, A. (2018). Microbial modulation of plant ethylene signaling: Ecological and Evolutionary Consequences, Microbiome . 6:52.
Rostami, A., Sadravi,  M., Rezaei, R. and Abdollahi, M. (2021). Biological control of Fusarium root rot of bean with two Trichoderma species and Pseudomonas fluorescens. Plant pathology science, 9(2): 234-240.
Saghaf, A. M., Aini, H., Zaman. H. and Hanif, M. (2013). Review of person re_identification techniques. The Institution of Enginnering and Technology, 6(8): 455_474.
 
Saravanakumar, D. and Samiyappan, R. (2007). ACC deaminase from Pseudomonas fluorescens mediated saline resistance in groundnut (Arachis hypogea) plants. Journal of Applied Microbiology, 120: 1283-1292.
Sharma, R. (2003). Barriers in using technology for education in developing countries. Singapore schools compututers and education, 41: 49_63.
Shaw, R. J. (1999). Soil salinity – electrical conductivity and chloride. In: Peverill KI, Sparrow LA, Reuter DJ (eds) Soil analysis: an interpretation manual. CSIRO Publishing, Melbourne. 129–146.
Smith, R. H., Hüberli, D., Sharma, D. L. and D’Antuono, M. F. (2019). Soil salinity exacerbates crown rot in wheat. Australasian Plant Pathology, doi.org/10.1007/s13313-019-00634-2.
Zhori, A., Meco, M., Brandl, H., and Bachofen, R. (2015). In situ chlorophyll fluorescence kinetics as a tool to quantify effects on photosynthesis in Euphorbia cyparissias by a parasitic infection of the rust fungus Uromyces pisi. BMC Research Notes, 8, 698.
 
BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 10, Issue 1
June 2022
Pages 89-95

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