Antagonistic activity of some epiphytic bacteria and actinomycetes against ice nucleation active bacteria on Rafsanjan pistachio trees

Document Type : Complete paper

Authors

1 Department of Plant Protection, Rafsanjan Branch, Islamic Azad University, Rafsanjan, Iran

2 Department of Plant Pathology, Islamic Azad University, Rafsanjan Branch,Rafsanjan ,Iran

Abstract

One of the aggravating factors in the amount of frost damage, is the presence of epiphytic ice nucleation bacteria on plant philosopher. Biological control of ice nucleation bacteria seems to be a promising option to reduce this type of frost damage. In this study, the antimicrobial capacity of epiphytic bacteria and actinomycetes was evaluated through growth inhibition of ice nucleation active bacteria (Pseudomonas fragi raf3) on pistachio in Rafsanjan. For this purpose, samples were collected from healthy pistachio leaves in Rafsanjan. Among the isolated bacteria and actinomycetes, two isolates (A4 and B52) with the highest inhibitory zone were subject to different biochemical and physiological tests. Results showed that the zone of inhibition growth of ice nucleation bacterium on Petri dish, was 20 mm and 14 mm for isolates A4 and B52 respectively. Analysis of morphological and biochemical characteristics showed that isolate A4 was noted to be Gram-positive and to form white, dry colonies similar to actinomycetes. It was able to degrade glucose and sucrose as a carbon source and able to produce lipase, protease and catalase enzymes. This isolate was able to grow in conditions with pH 5 and 7, as well as, showed 2, 5,7 and 10% (w/v) salt-salt (sodium chloride) tolerance. It was tetracycline-sensitive and penicillin-resistance. The other antagonist isolate, B52, had yellow colonies on nutrient agar media Gram-negative bacterium, catalase positive, oxidase negative, facultative anaerobic and lactose fermenting.

Keywords

References

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BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 10, Issue 1
June 2022
Pages 77-87

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