Genetic diversity of some important strains of Pseudomonas fluorescens and their effects on defense enzymes and growth parameters of bread wheat

Document Type : Complete paper

Authors

1 Department of Genetics and Plant Production, Faculty of Agriculture, Vali-e-Asr University, Rafsanjan, Iran

2 Department of Plant Protecion, Faculty of Agriculture, Vali-e-Asr University, Rafsanjan

3 Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University, Rafsanjan, Iran

Abstract

Pseudomonas fluorescens bacteria are the most important rhizosphere bacteria that have a positive effect on vegetative and biochemical traits of the plant. In this study, genetic diversity of 22 Pseudomonas fluorescens strains and their effect on defense enzymes and growth factors of wheat plant were performed in three stages of greenhouse, molecular and biochemical evaluation. In molecular evaluation, 12 RAPD primers were used to evaluate the genetic diversity of strains. Biochemical evaluation was performed in 4 stages of 0, 3, 6 and 9 days after inoculation on wheat to measure the defense enzymes peroxidase and phenylalanine ammonialyase. VUPF5 strain on wheat increased shoot and biological weight. RAPD-PCR separated strains with similar geographic location and similar host. Greenhouse, molecular, and biochemical groupings identified almost the top strains. Strains 680, 738, 50, 354, 49, 58 and VUPF5, which had a positive effect on molecular evaluation in one group, were shared with the superior group of enzymes. T26-2 and F-68 in OP-I15 and OP-H8 primers amplified 1300 bp and 1200 bp bands, respectively witch these bands can evaluated after be further studies as candidate bands in the expression of these enzymes in wheat.

Keywords

References

Abbass Z, Okon Y (1993) Plant growth promotion by Azotobacter paspali in the rhizosphere. Soil biology and biochemistry 25: 1075-1083.
Acosta M, Rodriguez-Lopez JN, Pendent MA (2002) Plant peroxidases. University of Murcia.
Anderson AJ, Guerra D (1985) Responses of bean to root colonization with Pseudomonas putida in a hydroponic system. Phytopathology 75: 992-995.
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1992) Short Protocols in Molecular Biology. Jonh Willey & Sons Inc., London.
Avis TJ, Gravel V, Antoun H, Tweddell RJ (2008) Multifaceted beneficial effects of rhizosphere microorganisms on plant health and productivity. Soil biology and biochemistry 40: 1733-1740.
Bais, HP, Prithiviraj B, Jha AK, Ausubel FM, Vivanco JM (2005) Mediation of pathogen resistance by exudation of antimicrobials from roots. Nature 434: 217-221.
Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical of Biochemistry 72: 248–254.
D'cunha GB, Satyanarayan V, Nair PM (1996) Purification of phenylalanine ammonialyase from rhodotorula glutinis. Journal of Phytochemistry 42: 17-20.
Diaz MH, Hauser AR (2010) Pseudomonas aeruginosa cytotoxin ExoU is injected into phagocytic cells during acute pneumonia. Infection and immunity 78: 1447-1456.
Guerinot ML (1991) Iron Uptake and metabolism in the rhizobia/legume symbioses. Plant and soil 130: 199-209.
Hoft M, Seong K, Jurkevitch E, Verstraete W (1991) Pyoverdin production by the plant growth beneficial Pseudomonas strain 7NSK 2: Ecological significance in soil. Plant and soil 130: 249-257.
Holt JG, Williams ST (1989) Bergey's manual of systematic bacteriology, Vol. 4. Lippincott Williams & Wilkins.
Kang BR, Yang KY, Cho BH, Han TH, Kim IS, Lee MC, Anderson AJ, Kim YC (2006) Production of indole-3-acetic acid in the plant-beneficial strain Pseudomonas chlororaphis O6 is negatively regulated by the global sensor kinase GacS. Current microbiology 52: 473-476.
Kloepper JW, Gutierrez-Estrada A, Mclnroy JA (2007) Photoperiod regulates elicitation of growth promotion but not induced resistance by plant growth-promoting rhizobacteria. Microbiology 53(2): 159-167.
Ktari S, Mnif B, Znazen A, Rekik M, Mezghani S, Mahjoubi-Rhimi F, Hammami A (2011) Diversity of β-lactamases in Pseudomonas aeruginosa isolates producing metallo-β-lactamase in two Tunisian hospitals. Microbial Drug Resistance 17: 25-30.
Kumar NR, Arasu VT, Gunasekaran P (2002) Genotyping of antifungal compounds producing plant growth-promoting rhizobacteria, Pseudomonas fluorescens. Current Science.
Mavrodi OV, McSpadden Gardener BB, Mavrodi DV, Bonsall RF, Weller DM, Thomashow LS (2001) Genetic diversity of phlD from 2, 4-diacetylphloroglucinol-producing Pseudomonas fluorescent spp. Phytopathology 91: 35-43.
Milbourne D, Meyer R, Bradshaw JE, Baird E, Bonar N, Provan J, Powell W, Waugh R (1997) Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato. Molecular breeding 3(2):127-36.
M'piga P, Belanger R, Paulitz T, Benhamou N (1997) Increased resistance toFusarium oxysporumf. sp. radicis-lycopersiciin tomato plants treated with the endophytic bacterium Pseudomonas fluorescensstrain 63-28. Physiological and Molecular Plant Pathology 50: 301-320.
Ping L, Boland W (2004) Signals from the underground: Bacterial volatiles promote growth in Arabidopsis. Trends in plant science 9: 263-266.
Pirdashti H, Mottaghian A, Tajick Ghanbary MA (2010) Response of growth characters and yield of wheat (Triticum aestivum L.) to co-inoculation of farmyard manure, Trichoderma spp. and Pseudomonas spp. Journal of Agronomy 2(3): 448-458 (In Persian).
Plewa MJ, Smith SR, Wagner ED. (1991) Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 247(1): 57-64.
Powell W, Morgante, M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular breeding 2: 225-238.
Prabhukarthikeyan SR, Umapathy K, Thiruvengadam R, Yadav MK (2010) Comparative analysis of genetic diversity among fluorescent pseudomonads using RAPD and ISSR fingerprinting. Research Journal of Biotechnology 14(7): 86-93.
Rayar JK, Arif M, Singh US (2015) Relative efficiency of RAPD and ISSR markers in assessment of DNA polymorphism and genetic diversity among Pseudomonas strains. African Journal of Biotechnology 14(13):1097-106.
Rezvan Beidokhti S, Dashtban A, Kafi M, Sanjani S (2009) Evaluating the effect of some Pseudomonas bacteria strains on wheat yield and its components at various levels of phosphorus fertilization. Journal of Agroecology 1(1): 33-40 (In Persian).
Souframanien J, Gopalakrishna TA (2004) Comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers. Theoretical and Applied Genetics 109: 1687-1693.
Todar M (2004) Pseudomonas aeruginosa in Web Review of Todar's Online Textbook of Bacteriology" The Good, the Bad, and the Deadly. Science Magazine 304: 1-12.
Van Peer R, Niemann G, Schippers B (1991) Induced resistance and phytoalexin accumulation in biological control of Fusarium wilt of carnation by Pseudomonas sp. strain WCS 417 r. Phytopathology 81: 728-734.
Ward Jr JH (1963) Hierarchical Grouping to Optimize an Objective Function. Journal of the American Statistical Association, 58: 236–244.
BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 10, Issue 1
June 2022
Pages 17-30

Files

Share

How to cite

Statistics