Evaluation of Some Plant Endophytic Fungi in Biological Control of Tomato Verticillium Wilt Disease

Document Type : Complete paper

Authors

1 Department of plant protection

2 Former student of plant pathology of plant protection department of univ. of Tehran

Abstract

Verticillium wilt disease caused by Verticillium dahliae is one of the most important diseases of tomato, for this reason, the control of this disease, including biological control is of serious interest. Endophytic fungi are one of the biological control agents that in addition to controlling plant diseases, improve growth in the host plant. In this study, the biocontrol potential of several isolates from plant endophytic fungi against tomato verticillium wilt was evaluated by dual culture methods in PDA, various laboratory and greenhouse test, respectively. Base on the results, three species of Nigrospora oryzae, Chaetomium globosum and Coniolariella limonispora selected from dual culture test, in addition to reducing the germination rate of microscelerotia of V. dahliae, they were able to produce cellulase and chitinase enzymes as well as the production of auxin. N. oryzae produced the highest amount of auxin with a production of 17.625 mg/l. In greenhouse studies and under conditions of artificial contamination in potting soil, N. oryzae endophytic fungus caused the greatest reduction in the severity of symptoms of verticillium wilt (67.61%) in tomato seedlings of sensitive Falat cultivar compared to the control. Comparison of the means of some growth indices showed that this fungus (N. oryzae) had a significant effect on increasing stem and root length, and fresh weight of the plant compared to other treatments.Nigrospora oryzae endophytic fungus was selected as the superior endophytic fungus in this study with the greatest effect on reducing the severity of V. dahliae disease and increasing stem and root length

Keywords

References

  1. Abdollahi-Aghdam Sh, Fotouhifar KhB (2017) Introduction of some endophytic fungi of sour cherry trees (Prunus cerasus) in Iran. Rostaniha 18(1): 77–94.
  2. Achouak W, Conrod S, Cohen V, Heulin, T (2004) Phenotypic variation of Pseudomonas brassicacearum as a plant root-colonization strategy. Molecular plant-microbe interactions 17 (8): 872-879.
  3. Akrami M, Yousefi, Z (2015) Biological control of Fusarium wilt of tomato (Solanum lycopersicum) by Trichoderma as antagonist fungi. Biological Forum 7 (1): 887-892.
  4. Daguerre Y, Siegel K, Edel-Hermann V, Steinberg C (2014) Fungal proteins and genes associated with biocontrol mechanisms of soil-borne pathogens: a review. Fungal biology reviews 28 (4): 97-125.
  5. Ding L, Yuan W, Peng Q, Sun H, Xu S (2016) Secondary metabolites isolated from the sponge-associated fungus Nigrospora oryzae. Chemistry of Natural Compounds 52 (5): 969-970.
  6. Dutta BK, (1981) Studies on some fungi isolated from the rhizosphere of tomato plants and the consequent prospect for the control of Verticillium wilt. Plant and Soil 63 209–216.
  7. Fravel DR, Roberts DP (1991) In situ evidence for the role of glucose oxidase in the biocontrol of Verticillium wilt by Talaromyces flavus. Biocontrol Science and Technology 1 (2): 91-99.
  8. Gao F, Dai CC, Liu XZ (2010) Mechanisms of fungal endophytes in plant protection against pathogens. African Journal of Microbiology Research 4 (13): 1346-1351.
  9. Gautam AK, Avasthi S (2019) Fungal endophytes: potential biocontrol agents in agriculture, In: Kumar A, et al. (Eds.), Role of plant growth promoting microorganisms in sustainable agriculture and nanotechnology. Elsevier Inc. pp. 240-298.
  10. Ghose TK (1987) Measurement of cellulase activities. Pure and applied Chemistry 59 (2): 257-268.
  11. Hsu SC, Lockwood JL (1975) Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Applied Environmental Microbiology 29 (3): 422-426.
  12. Jabnoun-Khiareddine H, Daami-Remadi M, Ayed FE, Mahjoub M (2009) Biological control of tomato Verticillium wilt by using indigenous Trichoderma The African Journal of Plant Science and Biotechnology 3 (1): 26-36.
  13. Jam Ashkezari S, Fotouhifar Kh-B (2017) Diversity of endophytic fungi of common yew (Taxus baccata) in Iran. Mycological Progress 16: 247–256.
  14. Klosterman SJ, Atallah ZK, Vallad GE, Subbarao KV (2009) Diversity, pathogenicity, and management of VerticilliumAnnual review of phytopathology 47: 39-62.‏
  15. Liu CH, Zou WX, Lu, H, Tan RX (2001) Antifungal activity of Artemisia annua endophyte cultures against phytopathogenic fungi. Journal of biotechnology 88 (3): 277-282.
  16. Mishra Y, Singh A, Batra A, Sharma MM (2014) Understanding the biodiversity and biological applications of endophytic fungi: a review. Journal of microbial biochemical technology science: 6 (8) 1-11.
  17. Masih EI, Paul B (2002) Secretion of β-1, 3-glucanases by the yeast Pichia membranifaciens and its possible role in the biocontrol of Botrytis cinerea causing grey mold disease of the grapevine. Current microbiology 44 (6): 391-395.
  18. Morid B, Hajmansoor S (2017) Assessment of tomato genotypes resistance to Verticillium and Fusarium wilt disease using molecular marker. Journal of the World of Microbes 10 (1): 80-93. (in Farsi).
  19. Murashima K, Kosugi A, Doi RH (2003) Synergistic effects of cellulosomal xylanase and cellulases from Clostridium cellulovorans on plant cell wall degradation. Journal of Bacteriology 185 (5): 1518-1524.
  20. Naraghi L, Heydari A, Rezaee S, Razavi M, Jahanifar H, Khaledi E (2010) Biological control of tomato Verticillium wilt disease by Talaromyces flavus. Journal of Plant Protection Research 50 (3): 360-365.
  21. Patten CL, Glick BR (2002) Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Applied Environmental Microbiology 68 (8): 3795-3801.
  22. Pawle G, Singh SK (2014) Antimicrobial, antioxidant activity and phytochemical analysis of an endophytic species of Nigrospora isolated from living fossil Ginkgo biloba. Current Research in Environmental and Applied Mycology 4 (1): 1-9.
  23. Portes CDS, Oliveira AVD, Simer P, Lunkes A M, Coelho AR (2013) Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus. Brazilian Archives of Biology and Technology 56(4): 619-627.
  24. Sica VP, Figueroa M, Raja HA, El-Elimat T, Darveaux BA, Pearce CJ, Oberlies NH (2016) Optimizing production and evaluating biosynthesis in situ of a herbicidal compound, mevalocidin, from ConiolariellaJournal of industrial microbiology & biotechnology 43 (8): 1149-1157.
  25. Sonawane A, Mahajan M, Renake S (2015) Antifungal activity of a fungal isolates against Pomegranate wilt pathogen Fusarium. International Journal of Current Microbiology and Applied Science 2: 48-57.
  26. Stosz SK, Fravel DR, Roberts DP (1996) In vitro analysis of the role of glucose oxidase from Talaromyces flavus in biocontrol of the plant pathogen Verticillium dahliae. Applied and Environmental Microbiology 62 (9): 3183-3186.
  27. Tjamos EC, Antoniou PP, Tjamos SE (2000) Implementation of soil solarization in Greece: conclusions and suggestions. Crop Protection 19 (8-10): 843-846.
  28. Tranier MS, Pognant-Gros J, Quiroz RDLC, González CNA, Mateille T, Roussos S (2014) Commercial biological control agents targeted against plant-parasitic root-knot nematodes. Brazilian Archives of Biology and Technology 57 (6): 831-841.
  29. Uppal AK, Hadrami A, Adam LR, Tenuta, M, Daayf F (2008) Biological control of potato Verticillium wilt under controlled and field conditions using selected bacterial antagonists and plant extracts. Biological control 44 (1): 90-100.
  30. Wang X, Wang C, Xie C, Yang X (2014) Advances in molecular mechanisms of Verticillium pathogenicity and plant resistance to Verticillium wilt. Journal of Henan Agricultural Sciences 43 (1): 1-6.
  31. Wang Y, Xu L, Ren W, Zhao D, Zhu Y, Wu X (2012) Bioactive metabolites from Chaetomium globosum L18, an endophytic fungus in the medicinal plant Curcuma wenyujin. Phytomedicine 19 (3-4): 364-368.
  32. Xiao CL, Subbarao KV (2000) Effects of irrigation and Verticillium dahliae on cauliflower root and shoot growth dynamics. Phytopathology 90 (9): 995-1004.
  33. Zheng Y, Xue QY, Xu, LL, Xu Q, Lu S, Gu C, Guo, JH (2011) A screening strategy of fungal biocontrol agents towards Verticillium wilt of cotton. Biological Control 56 (3): 209-216.

 

BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 9, Issue 2
September 2021
Pages 103-114

Files

Share

How to cite

Statistics