Using cheap by-products of food industry for cultivation of Paenibacillus polymyxa N179

Document Type : Complete paper

Authors

1 Assistant prof.

2 Department of plant protection,, Azarbaijan Shahid Madani University

3 Department of Plant protection, Azarbaijan Shahid Madani University

Abstract

In current research Paenibacillus polymyxa strain N179 was selected based on its previous performance in greenhouse and field condition, and cheap by-products of food industry was used to introduce a best medium for its high quality and low cost cultivation. The growth rate of this bacterium in various media was significantly different. In addition, antagonistic activity of cells from various media either in-vitro or in greenhouse condition was significantly different. The cells from M4 (10 g date pomace, 4 g sesame press cake) and M10 (4 g sesame press cake, 5 g starch) have shown highest inhibition against Rhizoctonia solani fungus. The resistance of cells from media against formulation process and viability during preservation period was assessed and it was found that cells from LB, M9, M7, and M2 media were the most resistant ones during preservation period. Moreover, efficacy of prepared formulations was assessed after three months in greenhouse condition and it was found that formulations made from M5 and LB were the most effective ones in controlling the disease. However, considering all of the results, M9 was shown the best performance.

Keywords


Agrios GN. (2005). Plant pathology. 5th edition. Elsevier academic press. Amesterdam.
Alizadeh M, Vasebi Y, Safaie N (2020) Microbial antagonists against plant pathogens in Iran: A review. Open Agriculture 5: 404–440.
Ashofteh F, Ahmadzadeh M, Fallahzadeh-Mamaghani V (2009) Effect of mineral components of the medium used to grow biocontrol strain UTPF61 of Pseudomonas fluorescens on its antagonistic activity against Sclerotinia wilt of sunflower and its survival during and after the formulation process. Journal of Plant Pathology 607–613.
Barratt BIP, Moran VC, Bigler F, Van Lenteren JC (2018) The status of biological control and recommendations for improving uptake for the future. BioControl 63: 155–167.
Costa E, Teixidó N, Usall J, Atarés E, Viñas I (2001) Production of the biocontrol agent Pantoea agglomerans strain CPA-2 using commercial products and by-products. Applied Microbiology and Biotechnology 56: 367–371.
Fallahzadeh-Mamaghani V, Golchin S, Shirzad A, Mohammadi H, Mohamadivand F (2021) Characterization of Paenibacillus polymixa N179 as a robust and multifunctional biocontrol agent. Biological Control 154: 104505.
Fallahzadeh V, Ahmadzadeh M. (2011) Fermentation thechnology of biological control agents. 2011, Tehran.
Ghasemi S, Ahmadzadeh M, Khodaian Cheghini F (2013) Culture medium designing for semi-industrial production of Bacillus subtilis UTB96. Biological control of pests and plant diseases 2: 149–160.
Heidari TF, Ahmadzadeh M, Moeinzadeh A (2011) Comparison of sugar beet and sugar cane molasses regarding their influence on production and efficiency of Pseudomonas fluorescens, the biocontrol agent of Sclerotinia sclerotiorum. Journal of sugar beet. 27(1): 39-52. (In persian)
Heydari A, Pessarakli M (2010) A review on biological control of fungal plant pathogens using microbial antagonists. Journal of biological sciences 10: 273–290.
Liu J, Li G, Sui Y (2017) Optimization of culture medium enhances viable biomass production and biocontrol efficacy of the antagonistic yeast, Candida diversa. Frontiers in microbiology 8: 2021.
Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P (2000) Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiology and molecular biology reviews 64: 548–572.
Schisler DA, Jackson MA, Bothast RJ (1991) Influence of nutrition during conidiation of Colletotrichum truncatum on conidial germination and efficacy in inciting disease in Sesbania exaltata. Phytopathology 81: 458–461.
Schisler DA, Slininger PJ, Behle RW, Jackson MA (2004) Formulation of Bacillus spp. for biological control of plant diseases. Phytopathology 94: 1267–1271.
Sharifi R, Ahmadzadeh M, Sharifi-Tehrani A, Fallahzadeh-Mamaghani V, Talebi-jahromi K (2008) Competition for iron uptake by fluorescent pseudomonads to control of Rhizoctonia solani kuhn causing agent of bean damping-off disease. Journal of Plant Protection 22(2); 183-195. (In perisan).
Shirzad A, Fallahzadeh-Mamaghani V, Pazhouhandeh M (2012) Antagonistic potential of fluorescent pseudomonads and control of crown and root rot of cucumber caused by Phythophtora drechsleri. The Plant Pathology Journal 28: 1–9.
Slininger PJ, Shea-Wilbur MA (1995) Liquid-culture pH, temperature, and carbon (not nitrogen) source regulate phenazine productivity of the take-all biocontrol agent Pseudomonas fluorescens 2-79. Applied microbiology and biotechnology 43: 794–800.
Stanbury PF, Whitaker A, Hall SJ (2013) Principles of fermentation technology. Elsevier. United Kingdom.