First isolation of Bdellovibrio sp from Water and Soil samples in Rafsanjan city

Heidari Taj Abadi, Fereshteh; Ahmadzadeh, Masoud; Salehi Juzani, Gholamreza; Khodaygan, Pejman

doi:10.22059/jbioc.2018.256406.229

First isolation of Bdellovibrio sp from Water and Soil samples in Rafsanjan city

Document Type : Complete paper

Authors

Fereshteh Heidari Taj abadi ¹

Masoud Ahmadzadeh ²

Gholamreza Salehi Juzani ³

Pejman Khodaygan ⁴

¹ Department of Plant Protection, University of Tehran, Iran

² Department of plant protection, university of Tehran-Iran.

³ Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII)

⁴ Department of plant protection. University of Vali-e-Asr of Rafsanjan

10.22059/jbioc.2018.256406.229

Abstract

Bdellovibrio bacteriovorus is an obligate predator of other Gram-negative bacteria. It grows in the periplasm of the prey cell and finally lyses them, so it has high potentials to be used in biocontrol of plant pathogenic bacteria. The objective of the present study was to isolate and characterize Bdellovibrios bacteria from soil and water samples of Rafsanjan (Kerman province) in Iran. To do this, after sampling, two Bdellovibrios isolates (Sar and Pj) were isolated by using Escherichia coli strain K12 as prey on the two layers agar. The isolates were identified by phenotypic and 16S rDNA PCR amplification and sequencing. The sequencing and phylogenetic studies showed that the strains have more than 99% similarity to B. bacteriovorus. Then, the host range and specificity of the strain were evaluated on 9 different Gram negative bacterial species. The results showed that the strains have predatory effects only on E. coli as prey.

Keywords

Predatory bacteria

Bdellovibrio bacteriovorus

prey range

biological control and 16S rDNA