A new record of insect pathogen on larva of Rosaceous longhorn beetle, Osphranteria coerulescens (Coleoptera: Cerambycidae)

Document Type : Complete paper

Authors

1 Ferdowsi University of Mashhad

2 Associate Prof. Ferdowsi University Of Mashhad

Abstract

The Rosaceous longhorn beetle (RLHB), Osphranteria coerulescens Redtenbacher (Coleoptera: Cerambycidae) is a significant pests of fruit orchard in Iran and it is costly
to control it. During an ongoing project on natural enemies of O. coerulescens in Mashhad region, North East of Iran, two isolates of entomopathogenic fungi (EPF) were isolated as natural pathogens on the larvae. The fungal isolates were identified based on morphological characters and then ITS gene sequences were used for inferring phylogenetic relationship. These isolates were identified as Beauveria bassiana sensu lato (Balsamo) Vuillemin (Hypocreales: Clavicipitaceae) and Metarhizium anisopliae sensu lato (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae). In the laboratory bioassay, potential virulence of entomopathogenic fungi against O. coerulescens larvae was conducted in various treatments. The larvae were susceptible to both EPF isolates, but the B. bassiana isolate caused significantly higher mortality than the M. anisopliae isolate. Application of EPF suspensions under special climatic conditions against in early life stages is thought to be promising for control of O. coerulescens.

Keywords

Main Subjects

References

Aquino Demuro, M., Elliotte, S., Moore, D., Parker, B., Skinner, M., Reid, W. and El bouhssini, M. 2004. Molecular characterisation of Beauveria bassiana isolates obtained from overwintering sites of Sunn Pests (Eurygaster and Aelia species). Mycological Research, 109: 294-306.
Behdad, E. 1984. Pests of fruit trees in Iran. Tehran, Iran, Plant Pests and Diseases Research       Institute, 254-259.
Bidochka, M. J. 2001. Monitoring the fate of biocontrol fungi. In Butt, T. M., Jackson, C. W. and          Magan, N. (Eds) Fungi as biocontrol agents: Progress, problems and potential. Wallingford, UK: CABI Publishing. pp. 193-218.
Borman, A., Szekely, A., Campbell, C. and Johnson, E. 2006. Evaluation of the viability of     pathogenic filamentous fungi after prolonged storage in sterile water and review of recent          published studies on storage methods. Mycopathologia, 161: 361–368.
Castrillo, L. A., Ugine, T. A., Filotas, M. J., Sanderson, J. P., Vandenberg, J. D. and Wraight, S.     P. 2008. Molecular characterization and comparative virulence of Beauveria bassiana             isolates (Ascomycota: Hypocreales) associated with    the greenhouse shorefly,                     Scatella tenuicosta (Diptera: Ephydrididae). Biological Control, 45: 154-162.
Chandler, D., Hey, D. and Reid, A.P. 1996. Sampling and occurrence of entomopathogenic fungi         and nematodes in UK          soils. Applied Soil Ecology, 5: 133-141.
Driver, F., Milner, R. J. and Trueman, J. W. H. 2000. A taxonomic revision of Metarhizium based on phylogenetic analysis of rDNA sequence data. Mycological Research, 104: 134–150.
Esmaeili, M. 1983. Important pests of fruit trees. Tehran, Iran, Sepehr Publishing Center, 176-     180.
Goettel, M. S. and Inglis, G. D. 1997. Fungi: Hyphomycetes. In . Lacey, L. (Eds) Manual of       techniques in insect pathology. San Diego, CA: Academic Press, pp. 213–249.
Goettel, M. S., Inglis, G. D. and Wraight, S.P. 2000. Fungi. In: Lacey, L. A. and Kaya, H. K.   (Eds) Field Manual of Techniques in Invertebrate Pathology. Kluwer Academic Press,    Dordrecht, the Netherlands, pp. 255–282.
Hall, T. A. 1999. Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Research,41: 95–98.
Humber, R. A. 2012b. Identification of entomopathogenic fungi. In Lacey L. A. (Eds) Manual of          Techniques in            Invertebrate Pathology. Academic Press, Amsterdam, the Netherlands, pp 151–187.
Inglis, G. D., Enkerli, J. and Goettel, M. S. 2012. Laboratory techniques used for           entomopathogenic fungi: Hypocreales. In:            Lacey, L. A. (Eds) Manual of Techniques in Invertebrate Pathology, 2 edition. Academic Press, Elsevier Science, pp. 18-53.
Jackson, M.  A., Dunlap, C.  A. and Jaronski, S. T. 2010. Ecological considerations in producing and formulating fungal entomopathogens for use in insect biocontrol. BioControl, 55: 129–145.
Jaronski, S. 2009. Ecological factors in the inundative use of fungal Entomopathogens.              BioControl, 55:159–185.
Keller, S. and Zimmerman, G. 1989. Mycopathogens of soil insects. In: Wilding, N., Collins, N.            M., Hammond, P. M. and Webbe, J. F. (Eds) Insect-Fungus Interactions. Academic        Press, London, UK, pp. 239-270.
Keller, S., Kessler, P. and Schweizer, C . 2003. Distribution of insect pathogenic soil fungi in    Switezerland with special reference to Beauveria brongniartii and Metarhizium                           anisopliae. Biocontrol, 48: 307–319.
Lin, L., wang, F. and Wei, D. 2011. Chlorimuron ethyl as a new selectable marker for disrupting            genes in the insect-pathogenic fungus Metarhizium robertsii. Journal of Microbiological         Methods, 87: 241–243.

Meyling, N. V. and Eilenberg,  J. 2007. Ecology of the entomopathogenic Beauveria bassiana   and Metarhizium anisopliae          in temperate agroecosystems: conservation biological            control. BiologicalControl, 43: 145–155.

Meyling, N. V., Lubeck, M., Buckley, E. P., Eilenberg, J. and Rehner, S. A., 2009. Community    composition, host range and genetic structure of the fungal entomopathogen Beauveria in             adjoining agricultural and seminatural habitats. Mycological Research, 18: 1282–1293.
Oliveira, I., Pereira, J. A., Bento, A. and Baptista, P. 2010. Viability of Beauveria bassiana      isolates after storage under     several preservation methods. Annals of Microbiology,         61:339-344
Patricia, H. S., Pedro, M. O. J. N., Talita, M. A., Daniele, S., Luis, F. A. A. & and, Fungaro, M.        H. P. 2008. Selection of Beauveria    bassiana isolates to control Alphitobius diaperinus.   Journal of Invertebrate Pathology, 97: 83–90.
Pérez-González, V. H., Guzmán-Franco, A. W., Alatorre-Rosas, R., Hernández-López, J.,    Hernández-López, A., Carrillo-Benítez, M. G. and Baverstock, J. 2014. Specific diversity      of the entomopathogenic fungi Beauveria and Metarhizium         in Mexican agricultural soils. Journal of Invertebrate Pathology, 119: 54–61.
Quesada-Moraga, E., Navas-Cortés, J. A., Maranhao, E. A. A., Ortiz-Urquiza, A. and Santiago-      Álvarez, C., 2007. Factors affecting the occurrence and distribution of entomopathogenic      fungi in natural and cultivated soils. Mycological Research, 111: 947– 966.
Reineke, A., Bischoff-Schaefer, M., Rondot, Y., Galidevara, S., Hirsch, J. and Uma Devi, K.            2014. Microsatellite markers to monitor a commercialized isolate of the       entomopathogenic fungus Beauveria bassiana in different environments: Technical  validation and first applications.Biological Control, 70: 1–8.
Riba, G., Bouvier-Fourcade, I. and Caudal, A. 1986. Isoenzymes polymorphism in Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) entomogenous fungi. Mycopathologia, 96: 161-169.
Senthil kumar, C. M., Jacob, T. K., Devasahayam, S., D silvia, S. and Krishna Kumar, N. K.           2015. Isolation and characterization of a Lecanicillium psalliotae isolate infecting   cardamom thrips (Sciothrips cardamomi) in India. BioControl,. 60: 363-373.
Shin, T. Y., Lee, W. W., Hyun Ko, S., Choi, J. B., Bae, S. M., Choi, J. Y., Lee, K. S., Je, Y. H.,         Jin, B. R. and Woo, D. S. 2013. Distribution and characterization of entomopathogenic          fungi from Korean soils. Biocontrol Science and Technology, 3: 288-304.
Steinwender, B. M., Enkerli, J., Widmer, F., Eilenberg, J., Thorup-Kristensen, K. and Meyling       N. V. 2014. Molecular diversity of the entomopathogenic fungal Metarhizium community             within an agroecosystem. Journal of Invertebrate Pathology, 123: 6-12.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. 2011. MEGA5:   molecular evolutionary genetics analysis using maximum likelihood, evolutionary          distance, and maximum parsimony methods. Molecular Biology and Evolution, 28: 2731-       2739.
White, G. F. 1927. A method for obtining infective nematode from cultures. Sciences, 66: 302-303.
White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of      fungal ribosomal RNA genes for phylogenetics. In Innis, M. A, Gelfand, D. H, Sninsky,         J. J, White, T. J. (Eds) PCR protocols: a guide to methods and        applications.    Academic Press, San Diego,USA, pp 315-322.
 
BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 8, Issue 1
August 2019
Pages 75-84

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