Effect of barley endophytic fungi against of two pathogenic fungi, Gaeumannomyces graminis and Pythium aphanidermatum using dual culture method

Document Type : Short paper

Authors

1 Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran

2 ِDepartment of soil science, College of Agriculture, Razi University, Kermanshah, Iran

Abstract

Endophytic fungi can be efficient biological control agents. There is great evidence that these fungi have protected host plants against root pathogens. In this study, antagonistic effect of 20 isolates of endophytic fungi of barley roots including; Alternaria sp. (six isolates), Microdochium bolleyi (two isolates), Fusarium redolens (four isolates), Fusarium tricinctum (two isolates), F. monliforme (one isolate), Epicoccum nigrum (four isolates) and Clonostachys rosea (four isolates) were evaluated against two pathogenic fungi; Pythium aphanidermatum and Gaeumannomyces graminis in vitro. The Results of the impact of endophytic fungal isolates on P. aphanidermatum, indicated that highest percentage of inhibition was related to A. alternata and Alternaria sp. with 59.48 and 57.24 % and the least effectiveness was related to Epicoccum nigrum and Fusarium redolens with 8.92 percent. The Results of the impact of endophytic fungal isolates on G. graminis, indicated that highest percentage of inhibition was related to F. tricinctum and Alternaria sp. with 71.66 and 72.22 percent inhibition and the isolate Pleosporales sp. indicated no significant difference with control.

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References

Ainsworth G, Bisby G (1961) A Dictionary of the Fungi. Commonwealth Mycological Institute Kew, England.
Barnett HL, Hunter BB (1998) Illustrated genera of imperfect fungi. vol Ed. 4. American Phytopathological Society (APS Press(.
De Cal A et al. (2009) Population dynamics of Epicoccum nigrum, a biocontrol agent against brown rot in stone fruit. Journal of Applied Microbiology 106:592-605.
Hall TA BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series, 1999. pp 95-98.
Larran S, Perelló A, Simón MR, Moreno V (2007) The endophytic fungi from wheat (Triticum aestivum L.) World Journal of Microbiology and Biotechnology 23:565-572.
Liang H, Xing Y, Chen J, Zhang D, Guo S, Wang C (2012) Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae). BMC Complement and Alternative Medicine 12: 238-243.
Likar M, Regvar M (2013) Isolates of dark septate endophytes reduce metal uptake and improve physiology of Salix caprea L. Plant and Soil 370:593-604 doi:10.1007/s11104-013-1656-6.
Lübeck M, Knudsen IMB, Jensen B, Thrane U, Janvier C, Jensen DF (2002) GUS and GFP transformation of the biocontrol strain Clonostachys rosea IK726 and the use of these marker genes in ecological studies Mycological Research 106:815-826 doi:10.1017/s095375620200607x
Macia-Vicente JG, Jansson HB, Mendgen K, Lopez-Llorca LV (2008) Colonization of barley roots by endophytic fungi and their reduction of take-all caused by Gaeumannomyces graminis var. tritici Canadian Journal of Microbiology 54:600-609 doi:10.1139/w08-047
Mahadevakumar S, Jayaramaiah K, Janardhana G (2015) First report of leaf spot disease caused by Epicoccum nigrum on Lablab purpureus in India Mycological Progress 14.
Mejía LC et al. (2008) Endophytic fungi as biocontrol agents of Theobroma cacao pathogens. Biological Control 46:4-14.
Nel B, Steinberg C, Labuschagne N, Viljoen A (2006) The potential of nonpathogenic Fusarium oxysporum and other biological control organisms for suppressing Fusarium wilt of banana. Plant Pathology 55:217-223
Porras-Alfaro A, Bayman P (2011) Hidden fungi, emergent properties: endophytes and microbiomes. Annual Review of Phytopathology 49:291-315 doi:10.1146/annurev-phyto-080508-081831.
Qadri M,  Johri S,  Shah BA,  Khajuria A, Sidiq T, Lattoo SK, Abdin MZ,  Riyaz-Ul-Hassan S (2013) Identification and bioactive potential of endophytic fungi isolated from selected plants of the Western Himalayas. SpringerPlus 2:8-22.
Rodriguez RJ, White JF, Jr., Arnold AE, Redman RS (2009) Fungal endophytes: diversity and functional roles. The New Phytologist 182:314-330 doi:10.1111/j.1469-8137.2009,02773x.
Rosa LH,  Gonçalves VN, Caligiorne RB, Alves TMA, Rabello A, Sales PA, Alvaro J. Romanha AJ, et al. (2010) Leishmanicidal, trypanocidal and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil. Brazilian Journal of Microbiology 41: 420-430
Waard M, Georgopoulos S, Hollomon D, Ishii H, Leroux P, Ragsdale N, Schwinn F (1993) Chemical control of plant diseases: problems and prospects. Annual Review of Phytopathology 31:403-421.
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics PCR protocols: a guide to methods and applications 18:315-322
Zhang W, Krohn K, Draeger S, Schulz B (2008) Bioactive isocoumarins isolated from the endophytic fungus Microdochium bolleyi. Journal of Natural Products 71:1078-1081.
 
 
 
 
BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 7, Issue 2
December 2018
Pages 153-158

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