Effect of some mineral elements on biocontrol efficiency of Bacillus pumilus INR7 against bean damping-off caused by Rhizoctonia solani

Document Type : Complete paper

Authors

1 Former M.Sc. Student, Department of Plant Pathology, Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran

2 Associate Professor, Department of Plant Pathology, Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran

3 Assistant Professor, Department of Plant Pathology, Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran

Abstract

Culture design for mass production of biocontrol agents is one of the main approaches in commercialization of biocontrol agents. In this work, effects of some micro-nutrients have been investigated on biomass production and efficacy of B. pumilus against R. solani, the causal agent of bean damping-off. Seven elements, Fe, Zn, Cu, Mo, Mn, Co and B have been used in three concentrations. Bacterial biomass increased significantly in medium ammended with maximum concentrations of Fe (36 and 72 µM) and Cu (7 and 14 µM) and minimum concentration of Mn (12.5 µM). Bacterial population decreased significantly in minimum and medium concentrations of Zn (35 and 70 µM) and maximum concentration of Cobalt (14 µM) compared to M1 basal medium. In greenhouse experiment, highest disease suppression achieved in medium concentration of Fe (36 µM) and maximum concentration of Cu (14 µM). In other hand, maximum concentration of Fe (72 µM), minimum concentration of Zn (35 µM), maximum concentration of Mn (50 µM) and medium concentration of Mo (8.1 µM) decreased efficiency of bacteria in suppression of bean damping-off, significantly. In conclusion, the optimum concentration was not the same for tested elements. Even in the case of individual element, there was difference between optimum concentration for biomass production and efficacy of bacteria in biological control of the plant disease.

Keywords

Main Subjects

References

Ardalan A, Abbasi S, Sharifi R (2015) Comparison of some commercial Bacillus strains on biological control of Bean damping- off caused by Rhizoctonia solani, Proceedings of the 8th Congress in Advances in Agricultural Research, University of Kurdestan, Sanandaj. (in Persian)
 Asaka O, Shoda M (1996) Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Applied and Environmental Microbiology 62: 4081-4085.
Ashofteh F, Ahmadzadeh M, Fallahzadeh-Mamaghani V (2009) Effect of mineral components of the medium used to grow biocontrol strain UTPF61 of Pseudomonas fluorescens on its antagonistic activity against Sclerotinia wilt of sunflower and its survival during and after the formulation process. Journal of Plant Pathology 607-613.
Blom D, Fabbri C, Eberl L, Weisskopf L (2011) Volatile-mediated killing of Arabidopsis thaliana by bacteria is mainly due to hydrogen cyanide. Applied and Environmental Microbiology 77: 1000-1008.
Bruggen AHCv, Finckh MR (2016) Plant diseases and management approaches in organic farming systems. Annual Review of Phytopathology 54: 25-54.
Buhr TL, McPherson DC, Gutting BW (2008) Analysis of broth-cultured Bacillus atrophaeus and Bacillus cereusspores. Journal of Applied Microbiology 105: 1604-1613.
Costa E, Teixidó N, Usall J, Atarés E, Viñas I (2001) Production of the biocontrol agent Pantoea agglomerans strain CPA-2 using commercial products and by-products. Applied Microbiology and Biotechnology 56: 367-371.
Djavaheri M, Mercado-Blanco J, Versluis C, Meyer JM, Van Loon LC, Bakker PAHM (2012) Iron-regulated metabolites produced by Pseudomonas fluorescens WCS374r are not required for eliciting induced systemic resistance against Pseudomonas syringae pv. tomato in Arabidopsis. Microbiology Open 1: 311-325.
Duffy B, Défago G (1995) Influence of cultural conditions on spontaneous mutations in Pseudomonas fluorescens CHA0. Phytopathology 85: 1146.
Duffy BK, Défago G (1999) Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Applied and Environmental Microbiology 65: 2429-2438.
Flores ER, Pérez F, de la Torre M (1997) Scale-up of Bacillus thuringiensis fermentation based on oxygen transfer. Journal of Fermentation and Bioengineering 83: 561-564.
Grey B, Steck TR (2001) Concentrations of copper thought to be toxic to escherichia coli can induce the viable but nonculturable condition. Applied and Environmental Microbiology 67: 5325-5327.
Gu X-B, Zheng Z-M, Yu H-Q, Wang J, Liang F-L, Liu R-L (2005) Optimization of medium constituents for a novel lipopeptide production by Bacillus subtilis MO-01 by a response surface method. Process Biochemistry 40: 3196-3201.
Jeong H, Choi S-K, Kloepper JW, Ryu C-M (2014) Genome sequence of the plant endophyte Bacillus pumilus INR7, triggering induced systemic resistance in field crops. Genome Announcements 2: e01093-01114.
Kilian M, Steiner U, Krebs B, Junge H, Schmiedeknecht G, Hain R (2000) FZB24® Bacillus subtilis–mode of action of a microbial agent enhancing plant vitality. Pflanzenschutz-Nachrichten Bayer 1: 1.
Lewis JA (1991) Formulation and delivery systems of biocontrol agents with emphasis on fungi, The Rhizosphere and Plant Growth. Springer Nature, pp. 279-287.
Mahadtanap S, Sanguanser M, Cutler RW, Sardsud V, Anuntalabh S (2007) Control of anthracnose caused by Colletotrichum musae on Curcuma alismatifolia Gagnep. using antagonistic Bacillus spp. American Journal of Agricultural and Biological Sciences 2: 54-61.
Milner JL, Raffel SJ, Lethbridge BJ, Handelsman J (1995) Culture conditions that influence accumulation of zwittermicin A by Bacillus cereus UW85. Applied Microbiology and Biotechnology 43: 685-691.
Ownley BH, Trigiano RN (2016) Plant pathology concepts and laboratory exercises. CRC Press.
Posada-Uribe LF, Romero-Tabarez M, Villegas-Escobar V (2015) Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production. Bioprocess and Biosystems Engineering 38: 1879-1888.
Safari Asl F, Rouhani H, Falahati Rastegar M, Jahanbakhsh V (2010) effect of C and N source on the growth and antifungal activity of Bacillus subtilis bs against Pythium aphanidermatum. Journal of Plant Protection 24: 53-61. (in Persian)
Sharifi R, Ahmadzadeh M, Sharifi Tehrani A, Fallahzadeh V (2006) Competition for iron uptake by fluorescent pseudomonads to control of Rhizoctonia solani kuhn causing agent of bean damping-off disease. Journal of Plant Protection 22: 183-195. (In Persian).
Sharifi R, Ryu CM (2017) Chatting with a tiny belowground member of the holobiome: communication between plants and growth-promoting rhizobacteria. Advances in Botanical Research 82: 135-160.
Slininger P, Jackson M (1992) Nutritional factors regulating growth and accumulation of phenazine 1-carboxylic acid by Pseudomonas fluorescens 2-79. Applied Microbiology and Biotechnology 37: 388-392.
Slininger PJ, Shea-Wilbur MA (1995) Liquid-culture pH, temperature, and carbon (not nitrogen) source regulate phenazine productivity of the take-all biocontrol agent Pseudomonas fluorescens 2-79. Applied Microbiology and Biotechnology 43: 794-800.
Smaldone GT, Revelles O, Gaballa A, Sauer U, Antelmann H, Helmann JD (2012) A global investigation of the Bacillus subtilis iron-sparing response identifies major changes in metabolism. Journal of Bacteriology 194: 2594-2605.
Weinberg ED (1969) Biosynthesis of secondary metabolites: roles of trace metals. Advances in Microbial Physiology 4: 1-44.
BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 6, Issue 2 - Serial Number 2
October 2017
Pages 187-195

Files

Share

How to cite

Statistics