A study of enzymes expression pattern involved in biologic control by some wild and mutant strains of Trichoderma viride

Document Type : Complete paper

Authors

1 Graduated Ph.D. Student, Plant Protection Department, Faculty of Plant production, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran

2 Assistant Professor, Plant Protection Department, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Alborz, Iran

3 Associate Professor, Plant Protection Department, Faculty of Plant production, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran

Abstract

This study was carried out to create mutations in the fungus Trichoderma viride by gamma radiation (the optimal dose of 250 Gy) and then to check the increase or decrease in expression of the cellulase and chitinase enzymes in the mutant strains with respect to the wild isolates. Protein profiles were analyzed by SDS-PAGE electrophoresis. Finally, proteome pattern of mutant strains Tv M21 of T. viride was evaluatedby colloidal chitin substrate and two-dimensional electrophoresis techniques and software ImageMaster 2D Platinum. Highest variability of cellulase enzyme production was observed in the mutant strain wild isolates in the Tv M21, Tv M10, Tv M16 and Tv M14 and the highest diversity in the production of chitinase enzyme in the mutant strain in the wild isolates Tv M21, Tv M4 and Tv M1, respectively. Proteomic pattern revealed that the protein spots of mutant strains in comparison to wild isolates were both quantitatively and qualitatively different. This finding demonstrates that mutations can lead to changes in patterns of secreted proteins, so that may improve the control potential of plant disease.

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