A study of enzymes expression pattern involved in biologic control by some wild and mutant strains of Trichoderma viride

Document Type : Complete paper

Authors

1 Graduated Ph.D. Student, Plant Protection Department, Faculty of Plant production, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran

2 Assistant Professor, Plant Protection Department, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Alborz, Iran

3 Associate Professor, Plant Protection Department, Faculty of Plant production, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran

Abstract

This study was carried out to create mutations in the fungus Trichoderma viride by gamma radiation (the optimal dose of 250 Gy) and then to check the increase or decrease in expression of the cellulase and chitinase enzymes in the mutant strains with respect to the wild isolates. Protein profiles were analyzed by SDS-PAGE electrophoresis. Finally, proteome pattern of mutant strains Tv M21 of T. viride was evaluatedby colloidal chitin substrate and two-dimensional electrophoresis techniques and software ImageMaster 2D Platinum. Highest variability of cellulase enzyme production was observed in the mutant strain wild isolates in the Tv M21, Tv M10, Tv M16 and Tv M14 and the highest diversity in the production of chitinase enzyme in the mutant strain in the wild isolates Tv M21, Tv M4 and Tv M1, respectively. Proteomic pattern revealed that the protein spots of mutant strains in comparison to wild isolates were both quantitatively and qualitatively different. This finding demonstrates that mutations can lead to changes in patterns of secreted proteins, so that may improve the control potential of plant disease.

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References

Abo-State MA, Hammad AI, Swelim M, Gannam RB (2011) Enhanced production of cellulase(S) by Aspergillus spp. isolate from agriculture wastes by solid state fermentation american-eurasian. Journal of Agriculture Environment Science 8: 402-410.
Abo-State MA (2004) High-level xylanase production by radio-resistant, thermophilic Bacillus megaterium and its mutants in solid-state fermentation. Egyptic Journal of Biotechnology 17: 119-137.
Adav SS, Chao LT, Sze SK (2012) Quantitative secretomic analysis of Trichoderma reesei strains reveals enzymatic composition for lignocellulosic biomass degradation. Molecular and Cellular Proteomics 10: 1-15.
Andersen N (2007) Enzymatic hydrolysis of cellulose: experimental and modeling studies. PhD. Thesis. Univercity of Technical Denmark 154Pp.
Anderson NL (2005) The roles of multiple proteomic platforms in a pipeline for new diagnostics. Molecular and Cellular Proteomics 4: 1441-1444.
Bailey MJ, Tahtiharju J (2003) Efficient cellulase production by Trichoderma reesei in continuous cultivationon lactose medium with a computer-controlled feeding strategy. Applied Microbiology Biotechnology 62(2-3): 156-62.
Bhadauria V, Popescu L, Zhao WS, Peng YL(2007) Fungal transcriptomics. Microbiological Research 162: 285-298.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Analytical Biochemistry 7: 248-54.
Chandra M, Kalra A, Sangwan N, Gaurav S (2009) Development of a mutant of Trichoderma citrinoviride for enhanced production of cellulases. Bioresource Technology 100: 1659-1662.
Dahiya N, Tewari R, Hoondal G (2006) Biotechnological aspects of chitinolytic enzymes: a review. Applied Microbiology and Biotechnology 71(6): 773-782.
Divne C, Ståhlberg J, Teeri TT, Jones TA(1998) High-resolution crystal structures reveal how a cellulose chain is bound in the 50 Å long tunnel of cellobiohydrolase I from Trichoderma reesei. Journal of Molcular Biological 275: 309-325.
El-Katany MH, Gudely M, Robra KH, Elnaghy MA, Gubitez GM(2001) Characterization of Chitinase and endo ‌β-1, 3 glucanase from Trichoderma harzianum Rifai T21 involved in control of the phytopathogen sclerotium rolfsii. Applied Microbiology and Biotechnology 562: 137-143.
Elad Y, Chet I, Henis Y(1985) Degradation of plant pathogenic fungi by Trichoderma harzianum. Canadian Journal of Microbiology 28: 719-725.
Emma WG, Simeon OK(2008) The use of Trichoderma harzianum and Trichoderma viride as potential biocontrol agent against peanut microflora and their effectiveness in reducing aflatoxin contamination of infected kernels. Biothencnology Asian Network for Scientific Informaiton 1-9.
Ershadfath F, Mohsenzade F, Banegad H(2013) A study of Confidor toxin on the growth ability of Trichoderma different species agreeable to toxin. Sixteenth National Conference of Environmental Health. Tabriz University of Medical Sciences and Health Services.
Fägerstam L, Håkansson U, Pettersson G, Andersson L (1977) Purification of three different cellulolutic enzymes from Trichoderma viride QM 9414 on alarge scale. In Proceedings of Bioconversion Symposium. (ed. T. Gohose). Indian Institute of Technology New Dehli 165-178.
Firmino AP, Ulhoa CJ, Sousa MV, Ferreira Filho EX, Ricart CA (2002) Involvement of G proteins and cAMP in the production of chitinolytic enzymes by Trichoderma harzianum. Brazilian Journal of Microbiology 33:169-173.
Fleming K, Gray DG, Matthews S (2001) Cellulose crystallites. Chemistry Europian Journal 7: 1831-1836.
Grinyer J, McKay M, Nevalainen H, Herbert BR (2004) Fungal proteomics: initial mapping of biological control strain Trichoderma harzianum. Current Genetics 45:163-9.
Gulya T, Rashid KY, Masirevic SM (1997) Sunflower diseases in Sunflower technology and production, A.A. Schneiter (Ed.), American Society of Agronomy, Crop Science Society of American, Soil Sience Society of America Madison Wisconsin 263-379.
HarighiM J, Motallebi M, Zamani MR (2006) Antifungal activity of heterologous expressed chitinase (Chit42) from Trichoderma atroviride PTCC5220. Iranian Journal of Biotechnology 4: 95-103. (in Persian (
Harman GE, Bjorkman T (1998) Potential and existing uses of Trichoderma and Gliocladium plant disease control and plant growth enhancement. In: Kubicek, C.P., Harman, G.E. (Eds.), Trichoderma and Gliocladium, Enzymes, Biological Control and Commercial Applications. Taylor and Francis London 2: 229-265.
Harman GE, Howell CR, Viterbo A, Chet I, Lorito M (2004) Trichoderma species-opportunistic, avirulent plant symbionts. Nature Review Microbiology 2:43-56.
Henrissat B (1991) A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochemistry 280(2): 309-316.
Hoell IA, Dalhus B, Heggset EB, Aspmo SI, Eijsink VGH (2006) Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes. Federation of European Biochemical Societies Journal 273: 4889-4900.
Howell CR, Stipanovic RD (1983) Gliovirin, a new antibiotic from Gliocladium virens, and its role in the biological control of Pythium ultimum. Journal of Microbiology 29: 321-324.
Jun H, Kieselbach T, Jönsson L (2011) Enzyme production by filamentous fungi: analysis of the secretome of Trichoderma reesei grown on unconventional carbon source. Microbial Cell Factories 10: 68.
Karlsson J, Saloheimo M, Siika-Aho M, Tenkanen M, Penttilä M,Tjerneld F (2001) Homologous expression and characterization of Cel61A (EG IV) of Trichoderma reesei. Europian Journal of Biochemistry 268(24): 6498-507.
Karlsson J, Siika-aho M, Tenkanen M, Tjerneld F (2002) Enzymatic properties of the low molecular mass endoglucanases Cel12A (EG III) and Cel45A (EG V) of Trichoderma reesei. Journal of Biotechnology 99(1): 63-78.
Kotchoni SO, Shonukan OO (2000) Regualtory mutations affecting the synthesis of cellulase. World Journal of Microbiology and Biotechnology 18: 487-491.
Kucuk C, Kvanc M (2003) Isolation of Trichoderma spp. and determination of their antifungal, biochemical and physiological features. Turkish Journal of Biology 27(4): 247-253.
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
Mach RL, Peterbauer CK, Payer K, Jaksits S, Woo SL, Zeilinger S, Kullnig CM, Lorito M, Kubicek CP (1999) Expression of two major chitinase genes of Trichoderma atroviride (T. harzianum P1) is triggered by different regulatory signals. Applied Environmental Microbiology 65: 1858-63.
Mandels M, Weber J, Parizek R (1971) Enhanced cellulase production by a mutant of Trichoderma viride. Applied Microbiology 21: 152-154.
Montealegre J, Valderrama L, Herrera R, Besoaín X, Pérez LM (2009) Biocontrol capacity of wild and mutant Trichoderma harzianum (Rifai) strains on Rhizoctonia solani 618: effect of temperature and soil type during storage. Electronic Journal of Biotechnology 12: 2-3.
Moradi R, Shahbazi S, Ahari- Mostafavi H, Ebrahimi MA (2015) To analyze the effects gamma irradiation on morphological characteristics and antagonistic Trichoderma harzianum. Journal of Nuclear Science and Technology 71: 96-104. (in Persian)
Peberdy JF (1990) Fungal cell wall a review. Biochemistry of cell walls and membranes in fungi. Heidelberg, Germany: Springer-Verlag 5–24.
Prabavathy VR, Mathivanan N, Sagadevan E, Murugesan K, Lalithakumari D (2006) Strain improvement for enhanced production of cellulase in Trichoderma viride. Enzyme Microbilogy and Technology 38(5): 719-723.
Sayyedasli N, Zamani MR, Matlabi M, Harighi MJ (2004) Study of chitinase enzyme produce in Trichoderma. Iranian Journal of Biology 17(3): 227-233. (in Persian)
ShahbaziS, Ahari Mostafavi H, Mirmajlesi SM (2012) Assessing enzymes and molecular markers of Trichoderma mutants Endochitinases with specific STS. Iran's Third National Conference on Agricultural Biotechnology. Mashhad Ferdowsi University of Mashhad. (in Persian)
Shahbazi S, Ahari Mostafavi H, Ebrahimi MA, Askari H, Mirmajlesi SM, Karimi M (2013) Increased activity of chitinase gene from the fungus Trichoderma harzianum induced mutation using gamma rays. Journal of Biotechnology Crop Plants 5: 40-33. (in Persian)
Shoemaker SP, Brown RD (1978) Enzymatic activities of endo-1,4-h-D-glucanases purified from Trichoderma viride. Biochimestry and Biophysiology 523: 133-146.
Shoemaker SP, Watt K, Tsitovsky G, Cox R (1983) Characterisation and properties of cellulases purified from Trichoderma reesei strain L27. Biology and Technology 1: 687-690.
Suarez B, Rey M, Castillo P, Monte E, Llobell A (2004) Isolation and characterization of PRA1, a trypsin-like protease from the biocontrol agent Trichoderma harzianum CECT 2413 displaying nematicidal activity. Applied Microbiology and Biotechnology 65: 46-55.
Szekeres A, Kredics L, Antal Z, Kevei F, Manczinger L (2004) Isolation and characterization of protease overproducing mutants of Trichoderma harzianum. FEMS Microbiology Letter 233: 215-222.
Tseng YH, Kokkotou E, Schulz TJ, Huang TL, Winnay JN, Taniguchi CM, Tran T, Suzuki R, Espinoza DO, Yamamoto Y, Ahrens MJ, Dudley AT, Norris AW, Kulkarni RN, Kahn R (2008) New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure. Nature 454: 1000-1004.
Villena GK, Gutierrez-Correa M (2006) Production of cellulase by Aspergillus nigerbiofilms developed on polyester cloth. Letter Applied Microbiology 43(3): 226-62.
Viswanathan R, Premkumari SM, Sundar AR, Kathiresan T (2006) Cloning partial endochitinase cDNA of Trichoderma harzianumantagonistic to Colletotrichum falcatum causing red rot of sugarcane. Current Science 91: 951-956.
Westermeier R (2005) Electrophoresis in practice: a guide to methods and applications of DNA and differential protein separations. Fourth ed. Willey-VCHPublications.
Wey TT, Hseu TH, Huang L (1994) Molecular cloning and sequence analysisof the cellobiohydrolase I gene from Trichoderma koningii G-39. Current Microbiology 28: 31-39.
Xu F, Wang J, Chen S, Qin W, Yu Z, Zhao H, Xing X, Li H (2011) Strain improvement for enhanced production of cellulase in Trichoderma viride. Applied Biochemistry and Microbiology 47: 53-58.
BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 6, Issue 2 - Serial Number 2
October 2017
Pages 139-153

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