In vitro inhibitory effects of five strains of Trichoderma on the growth of Fusarium oxysporum f.sp. dianthi

Document Type : Short paper

Authors

1 M.Sc. Student in Plant Pathology, Department of Plant Protection, Faculty of Agriculture, University of Shahrekord, Shahrekord, Iran

2 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, University of Shahrekord, Shahrekord, Iran

3 Associate Professor, Department of Plant Protection, Faculty of Agriculture, University of Shahrekord, Shahrekord, Iran

Abstract

Carnation (Dianthus caryophyllus L.) is one of the four major exporting cut flowers from Iran. Carnation wilt caused by Fusarium oxysporum f.sp. dianthi is considered the most destructive diseases of plant in most carnation growing regions of the world . To investigate the possibility of using biological control, the growth inhibitory effects of five isolates of Trichoderma, including three isolates of Trichoderma harzianum obtained from Karaj (Th1), Shahrekord (Th2) and Hamedan (Th3), and two isolates T. virens (Tv) and T. atroviride (Ta) from Hamedan, studied in vitro on the F. oxysporum f.sp. dianthi isolated from Mahalat Carnation. The greatest growth inhibition in dual culture test was occurred with the Th3 isolate and the lowest inhibition observed by Ta. Culture extracts of Th1 isolate caused maximum inhibition of pathogen growth, while that of Th3 isolate showed minimal impact on its growth. Bioassay for production of antagonist volatile metabolites showed that the most growth inhibition was observed with the Ta isolate the least effective was recorded with Th3 isolate.

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References

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