Antifungal activity of medicinal plant essential oils against Mycogone perniciosa, causal agent of wet bubble and their effects on button mushroom

Document Type : Complete paper

Authors

1 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

2 Former M.Sc. Student, Department of Horticultural Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

3 Associate Professor, Department of Plant Protection, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

4 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

One of the most common pathogenic diseases of the button mushroom in Iran is wet bubble (Mycogone perniciosa). In this study, in order to develop a biological control system and production of healthy crop, the antifungal effect of aromatic medicinal plants were assayed as a safe replacement for chemical fungicides. M. perniciosa was isolated from infected A. bisporus cv. Sylvan 737. In order to evaluate the biocontrol possibility, the effect of six medicinal plant essential oils including garlic, horsemint, oregano, lavender, kotschyam thyme and eucalyptus were tested against M. perniciosa and evaluated their effects on button mushroom mycelium at 100, 500, 1000, 1500 and 2000 µl l-1 in vitro. Based on the results, kotschyam thyme and oregano were more toxic and minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) for M. perniciosa were 300 and 500 µl l-1, respectively. Interestingly, among the investigated essential oils, garlic oil showed a fungicidal effect on M. perniciosa at a lower concentration (MFC=1000 µl l-1) than button mushroom (MFC=1400 µl l-1). In general, garlic and lavender essential oils were found as the most effective and weakest oils against M. perniciosa, respectively. GC-MS analysis demonstrated that Trisulfide, di-2-propenyl, Piperitenone oxide,Thymol,1,8-Cineole, Thymol and Spathulenol were the main components of the above essential oils, respectively. It seems that garlic oil can be considered as a good alternative in biological control of wet bubble diseaseaccording to this research and considering consumerist tastes after additional researches.

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References

Ahmad A, Khan A, Akhtar F, Yousuf S, Xess I, Khan LA, Manzoor N (2011) Fungicidal activity of thymol and carvacrol by disrupting ergosterol biosynthesis and membrane integrity against Candida. European Journal of Clinical Microbiology and Infectious Diseases 30: 41-50.
Amiri H (2012) Essential oils composition and antioxidant properties of three Thymus species. Evidence-Based Complementary and Alternative Medicine, Article ID 728065, 8 pages doi:10.1155/2012/728065.
Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils – a review. Food and Chemical Toxicology 46: 446-475.
Bozovic M, Pirolli A, Ragno R (2015) Mentha suaveolens Ehrh. (Lamiaceae) essential oil and its main constituent piperitenone oxide: biological activities and chemistry. Molecules 20: 8605-8633.
Calvo-Gomez O, Morales-Lopez J, Lopez MG (2004) Solid-phase microextraction-gas chromatographic-mass spectrometric analysis of garlic oil obtained by hydrodistillation. Journal of Chromatography A 1036: 91-93.
Cantore PL, Iacobellis NS, De Marco A, Capasso F, Senatore F (2004) Antibacterial activity of Coriandrum sativum L. and Foeniculum vulgare Miller var. vulgare (Miller) essential oils. Journal of Agricultural and Food Chemistry 52: 7862-7866.
Chen S, Oh SR, Phung S, Hur G, Ye JJ, Kwok SL, Shrode GE, Belury M, Adams LS, Williams D (2006) Anti-aromatase activity of phytochemicals in white button mushrooms (Agaricus bisporus). Cancer Research 66: 12026-12034.
Darvishi E, Omidi M, Bushehri AA, Golshani A, Smith ML (2013) Thymol antifungal mode of action involves telomerase inhibition. Medical Mycology 51: 826-834.
D’auria FD, Tecca M, Strippoli V, Salvatore G, Battinelli L, Mazzanti G (2005) Antifungal activity of Lavandula angustifolia essential oil against Candida albicans yeast and mycelial form. Medical Mycology 43: 391-396.
Davari M, Ezazi R (2016)Study on the effects of four medicinal plant essential oils and two Trichoderma species on biocontrol of grapefruit rot fungi. Biological Control of Pests and Plant Diseases 5: 1-12.
Delaquis PJ, Stanich K, Girard B, Mazza G (2002) Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. International Journal of Food Microbiology 74: 101-109.
Elaissi A, Rouis Z, Ben Salem NA, Mabrouk S, Ben Salem Y, Bel Haj Salah K, Aouni M, Farhat F, Chemli R, Harzallah-Skhiri F, Larbi Khouja M (2012) Chemical composition of 8 Eucalyptus species' essential oils and the evaluation of their antibacterial, antifungal and antiviral activities. BMC Complementary and Alternative Medicine 12: 81.
Elshafie HS, Mancini E, Sakr S, De Martino L, Mattia CA, De Feo V, Camele I (2015) Antifungal activity of some constituents of Origanum vulgare L. essential oil against postharvest disease of peach fruit. Journal of Medicinal Food 18: 929-934.
Farzaneh M, Kiani H, Sharifi R, Reisid M, Hadiana J (2015) Chemical composition and antifungal effects of three species of Satureja (S. hortensis, S. spicigera, and S. khuzistanica) essential oils on the main pathogens of strawberry fruit. Postharvest Biology and Technology 109: 145-151.
Figueiredo AC, Barroso JG, Pedro LG, Scheffer JJC (2008) Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal 23: 213-226.
Fournomiti M, Kimbaris A, Mantzourani I, Plessas S, Theodoridou I, Papaemmanouil V, Kapsiotis I, Panopoulou M, Stavropoulou E, Bezirtzoglou EE, Alexopoulos A (2015) Antimicrobial activity of essential oils of cultivated oregano (Origanum vulgare), sage (Salvia officinalis), and thyme (Thymus vulgaris) against clinical isolates of Escherichia coli, Klebsiella oxytoca, and Klebsiella pneumonia. Microbial Ecology in Health & Disease 26: 23289. http://dx.doi.org/10.3402/mehd.v26.23289.
Gavanji S, Mohammadi E, Larki B, Bakhtari A (2014) Antimicrobial and cytotoxic evaluation of some herbal essential oils in comparison with common antibiotics in bioassay condition. Integrative Medicine Research 3: 142-152.
Glamoclija J, Sokovic M, Grubisic D, Vukojevic J, Milinekovic I, Ristic M (2009) Antifungal activity of Critmum maritimum essential oil and its components against mushroom pathogen Mycogone perniciosa. Chemistry of Natural Compounds 45: 96-97.
Gulluce M, Sahin F, Sokmen M, Ozer H, Daferera D, Sokmen A (2007) Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food Chemistry 103: 1449-1456.
Hussain AI, Anwar F, Nigam PS, Ashraf M, Gilani AH (2010) Seasonal variation in content, chemical composition and antimicrobial and cytotoxic activities of essential oils from four Mentha species. Journal of the Science of Food and Agriculture 90: 1827-1836.
Iqbal T, Hussain AI, Chatha SAS, Naqvi SAR, Bokhari TH (2013) Antioxidant activity and volatile and phenolic profiles of essential oil and different extracts of wild mint (Mentha longifolia) from the Pakistani flora. Journal of Analytical Methods in Chemistry, Article ID 536490, 6 pages, http://dx.doi.org/10.1155/2013/536490.
Kim JW, Kim YS, Kytung KH (2004) Inhibitory activity of essential oils of garlic and onion against bacteria and yeasts. Journal of Food Protection 67: 499-504.
Kordali S, Cakir A, Ozer H, Cakmakci R, Kesdek M, Mete E (2008) Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresource Technology 99: 8788-8795.
Küçükbay FZ, Kuyumcu E, Çelen S, Azaz AD, Arabaci T (2014) Chemical composition of the essential oils of three Thymus taxa from Turkey with antimicrobial and antioxidant activities. Records of Natural Products 8: 110-120.
Kunicka-Styczyn´ ska A, Sikora M, Kalemba D (2009) Antimicrobial activity of lavender, tea tree and lemon oils in cosmetic preservative systems. Journal of Applied Microbiology 107: 1903-1911.
Li WR, Shi QS, Dai HQ, Liang Q, Xie XB, Huang XM, Zhao GZ, Zhang LX (2016) Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans. Scientific Reports 6: 22805-22813.
Maffei M (1988) A chemotype of Mentha longifolia (L.) Hudson particularly rich in piperitenone oxide. Flavour and Fragrance Journal 3: 23-26.
Mancini E,Cameleb I,Elshafie HS,De Martino L,Pellegrino C,Grulova D,De Feo V (2014) Chemical composition and biological activity of the essential oil of Origanum vulgare ssp. hirtum from different areas in the southern Apennines (Italy). Chemistry & Biodiversity 11: 639-651.
Marchese A, Orhan IE, Daglia M, Barbieri R, Lorenzo AD, Nabavi SF, Gortzi O, Izadi M, Nabavi SM (2016) Antibacterial and antifungal activities of thymol: a brief review of the literature. Food Chemistry, doi: http://dx.doi.org/10.1016/j.foodchem.2016.04.111
Marino M, Bersani C, Comi G (2001) Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. International Journal of Food Microbiology 67: 187-195.
Mazooji A, Salimpour F, Danaei M, Akhoondi Darzikolaei S, Shirmohammadi K (2012) Comparative study of the essential oil chemical composition of Thymus kotschyanus Boiss. & Hohen var. kotschyanus from Iran. Annals of Biological Research 3: 1443-1451.
Mnayer D, Fabiano-Tixier AS, Petitcolas E, Hamieh T, Nehme N, Ferrant C, Fernandez X, Chemat F (2014) Chemical composition, antibacterial and antioxidant activities of six essentials oils from the Alliaceae family. Molecules 19: 20034-20053.

Moghaddam M, Farhadi N (2015) Influence of environmental and genetic factors on resin yield, essential oil content and chemical composition of Ferula assa-foetida L. populations. Journal of Applied Research on Medicinal and Aromatic Plants 2: 69-76.

Mohammadi Goltapeh E, Pourjam E (2005) Principles of mushroom cultivation. Tarbiat Modares University Press, 626p. In Persian.
Mota KSL, Pereira FO, Oliveira WA, Lima IO, Lima EO (2012) Antifungal activity of Thymus vulgaris L. essential oil and its constituent phytochemicals against Rhizopus oryzae: interaction with ergosterol. Molecules 17: 14418-33.
Pina-Vaz C, Gonçalves Rodriguez A, Pinto E, Costa-de-Oliveira S, Tavares C, Salgueiro L,Cavaleiro C, Gonçalves MJ, Martinez-de-Oliveira J (2004) Antifungal activity of Thymus (oils and their major compounds). Journal of the European Academy of Dermatology and Venereology 18: 73-78.
Pinto E, Goncalves MJ, Hrimpeng K, Pinto J, Vaz S, Vale-Silva LA, Cavaleiro C, Salgueiro L (2013) Antifungal activity of the essential oil of Thymus villosus subsp. lusitanicus against Candida, Cryptococcus, Aspergillus and dermatophyte species. Industrial Crops and Products 51: 93-99.
Rao A, Zhang Y, Muend S, Rao R (2010) Mechanism of antifungal activity of terpenoid phenols resembles calcium stress and inhibition of the TOR pathway. Antimicrobial Agents and Chemotherapy 54: 5062-5069. 10.1128/AAC.01050-10.
Russo M, Galletti GC, Bocchini P, Carnacini A (1998) Essential oil chemical composition of wild populations of Italian oregano spice (Origanum vulgare ssp. hirtum (Link) Ietswaart): a preliminary evaluation of their use in chemotaxonomy by cluster analysis. 1. Inflorescences. Journal of Agricultural and Food Chemistry 46: 3741-3746.
Sánchez MA, Turina AV, García DA, Nolan MV, Perillo MA (2004) Surface activity of thymol: implications for an eventual pharmacological activity. Colloids and Surfaces B: Biointerfaces 34: 77-86.
Shahriar A (2013) Identification of pathogenic fungi in mushroom production units in Ardabil province. M.Sc Thesis, University of Mohaghegh Ardabili, Ardabil, Iran. 
Sharma MV, Sagar A, Joshi M (2015) Study on antibacterial activity of Agaricus bisporus (Lang.) Imbach. International Journal of Current Microbiology and Applied Science 4: 553-558.
Sokovic M, Van Griensven LJLD (2006) Antimicrobial activity of essential oils and their components against the three major pathogens of the cultivated button mushroom, Agaricus bisporus. European Journal of Plant Pathology 116: 211-224.
Tanovic B, Potocnik I, Stanisavljevic B, Dordevic M, Rekanovic E (2006) Response of Verticillium fungicola var. fungicola, Mycogone perniciosa and Cladobotyum sp. mushroom pathogens to some essential oils. Pesticides and Phytomedicine 21: 231-237.
Tsao S, Yin M (2001) In vitro antimicrobial activity of four diallyl sulphides occurring naturally in garlic and Chinese leek oils. Journal of Medical Microbiology 50: 646-649.
Yang D, Michel L, Chaumont JP, Millet-Clerc J (2000) Use of caryophyllene oxide as an antifungal agent in an in vitro experimental model of onychomycosis. Mycopathologia 148: 79-82.
Yazgi M, Awad D, Jreikous B (2015) Screening of the antifungal activity of plant Mentha longifolia crude extracts against two fungi Alternaria citri and Fusarium moniliforme. Journal of Entomology and Zoology Studies 3: 359-364.
Zuzarte M, Goncalves MJ, Cavaleiro C, Cruz MT, Benzarti A, Marongiu B, Maxia A, Piras A, Salgueiro L (2013) Antifungal and anti-inflammatory potential of Lavandula stoechas and Thymus herba-barona essential oils. Industrial Crops and Products 44: 97-103.

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