Effect of biofilm by plant probiotic rhizobacteria on root colonization and growth of wheat

Document Type : Complete paper

Author

Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran

Abstract

A total of 11 probiotic strains of Bacillus subtilis were used to evaluate the potential of biofilm formation and wheat root colonization. Experiments carried out in a completely randomized design with four replications. According to results of this study, biofilm formation and biosurfactants production showed a high diversity in studied rhizobacteria, B3, B1 and B4 strains produced the maximum amount of mentioned metabolites. Evaluation of root colonization was performed using rifampicin and nalidixic acid resistant mutants. B4 and B3 with 4.41×107 cfu/gand 4.35×107 cfu/g had the highest root colonization compared to other strains. Correlation of 0.74 and 0.80 was obtained between biofilm formation and biosurfactants production with wheat root colonization, respectively. In another part of this study, the effects of studied strains were evaluated on wheat plant growth factors. All probiotic rhizobacteria strains increased the length of roots and aerial part of wheat and most of them increased dry weight of roots and aerial part. Based on the findings of this research, strains with high potential in biofilm formation and biosurfactants production were colonized wheat root very well and most of studied strains showed the effect of increasing on wheat growth factors.

Keywords

Main Subjects

References

Afsharmanesh H, Ahmadzadeh M (2016) The Iturin lipopeptides as key compounds in antagonism of Bacillus subtilis UTB96 toward Aspergillus flavus. Biological Control of Pests and Plant Diseases 5: 79-95. (in Persian)
 Ahmadzadeh M (2013) Biological control of plant diseases, plant probiotic bacteria. University of Tehran Press, Iran. 479 pp. (in Persian)
Ashtar HN, Zahir ZA, Arshad M (2004) Screening rhizobacteria for improving the growth, yield and oil content of canola (Brassica napus L.). Australian Journal of Agriculture Research 55: 187-194.
Bais HP, Fall R, Vivanco JM (2004) Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production. Plant Physiology 134: 307-319.
Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis 6: 71-79.
Berg G, Kurze S, Buchner A, Wellington EM, Smalla K (2000) Successful strategy for the selection of new strawberry-associated rhizobacteria antagonistic to Verticillium wilts. Canadian Journal of Microbiology 46: 1128-1137.
Boyd CD, Smith TJ, El-Kirat-Chatel S, Newell PD, DufrêneYF, O’Toole GA (2014) Structural features of the Pseudomonas fluorescens biofilm adhesin LapA required for LapG-dependent cleavage, biofilm formation and cell surface localization. Journal of Bacteriology 196: 2775-2788.
Branda SS, Vik A, Friedman L, Kolter R (2005) Biofilms: the matrix revisited. Trends Microbiology 13: 20-26.
Cherif-Silini H, Silini A, Yahiaoui B, Ouzari I, Boudabous A (2016) Phylogenetic and plant growth promoting characteristics of Bacillus isolated from the wheat rhizosphere. Annals of Microbiology 66: 1087-1097.
Damalas CA, Koutroubas SD (2016) Farmers’ exposure to pesticides: toxicity types and ways of prevention. Toxics 4: 1; Doi: 10.3390/toxics4010001.
Egamberdieva D (2010) Growth response of wheat cultivars to bacterial inoculation in calcareous soil. Plant, Soil and Environment 56: 570-573.
Ellis RJ, Timms-Wilson TM, Bailey MJ (2000) Identification of conserved traits in fluorescent pseudomonads with antifungal activity. Environmental Microbiology 2: 274-284.
Epstein AK,Pokroy B, Seminara A,Aizenberg J (2011) Bacterial biofilm shows persistent resistance to liquid wetting and gas penetration. PNAS 108: 995-1000.
Hsieh F, Li M, Lin T, Kao S (2004) Rapid detection and characterization of surfactin producing Bacillus subtilis and closely related species based on PCR. Current Microbiology 49: 186-191.
Johansson PM, Johnsson L, Gerhardson B (2003) Suppression of wheat-seedling diseases caused by Fusarium culmorum and Microdochium nivale using bacterial seed treatment. Plant Pathology 52: 219-227.
Kamilova F, Validov S, Azarova T, Mulders I, Lugtenberg B (2005) Enrichment for enhanced competitive plant root tip colonizers selects for a new class of biocontrol bacteria. Environmental Microbiology 7: 1809-1817.
Karnwal A (2012) Screening of plant growth–promoting rhizobacteria from maize (Zea mays) and wheat (Triticum aestivum).African Journal of Food, Agriculture, Nutrition and Development 12: 6171-6185.
Kearns DB (2008) Division of labour during Bacillus subtilis biofilm formation. Molecular Microbiology 67: 229-231.
Kempf HJ, Wolf G (1989) Erwinia herbicola as a biocontrol agent of Fusarium culmorum and Puccinia recondite f. sp. tritici on wheat. Phytopathology 79: 990-994.
Khezri M, Ahmadzadeh M, Salehi-Jouzani Gh, Behboudi K, Ahangaran A, Mousivand M, Rahimian H (2011)Characterization of some biofilm-forming Bacillus subtilis and evaluation of their biocontrol potential against Fusarium culmorum. Journal of Plant Pathology 93: 373-382.
Khezri M (2016) Influence of some environmental and nutritional conditions on biofilm formation of probiotic Bacillus subtilis strains. Biological Control of Pests and Plant Diseases 4: 157-165. (in Persian)
Khezri M, Ahmadzadeh M, Salehi Jouzani Gh, Sharifi R (2016)A new gene involving in biofilm formation of Bacillus subtilis. 11: 245-259. (In Persian).
Kinsella K, Schulthess CP, Morris TF, Stuart JD (2009)Rapid quantification of Bacillus subtilis antibiotics in the rhizosphere. Soil Biology and Biochemistry 41: 374-379.
Kobayashi K (2007) Bacillus subtilis pellicle formation proceeds through genetically defined morphological changes. Journal of Bacteriology 189: 4920-4931.
Kraus J, Loper JE (1995) Characterization of a genomic region required for production of the antibiotic pyluteorin by the biological control agent Pseudomonas fluorescens Pf-5. Applied and Environmental Microbiology 61: 849-854.
Majeed A, Kaleem Abbasi M, Hameed S, Imran A, Rahim N (2015) Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Frontiers in Microbiology 6: 198; Doi: 10.3389/fmicb.2015.00198.
Mansouripour SM, Alizadeh A, Safaei N (2008)Biocontrol ability and population dynamics of bacterial antagonists against Sclerotinia sclerotiorum in canola. Iranian Journal of Plant Pathology 44: 233-251.
Molina MA, Ramos JL, Urgel ME (2003) Plant-associated biofilms. Review in Environmental Science and Biotechnology 2: 99-108.
Morikawa M, Kagihiro S, Haruki M, Takano K, Branda S, Kolter R, Kanaya S (2006) Biofilm formation by a Bacillus subtilis strain that produces γ-polyglutamate. Microbiology 152: 2801-2807.
Morris C, Monier JM (2003) The ecological significance of biofilm formation by plant-associated bacteria. Annual Review of Phytopathology 41: 429-453.
Nagórska K, Hinc K, Stauch MA, Obuchowski M (2008) Influence of the sigmaB stress factor and yxaB, the gene for a putative exopolysaccharide synthase under sigmaB control, on biofilm formation. Journal of Bacteriology 190: 3546-3556.
Nasraoui B, Hajlaoui MR, Aïssa AD, Kremer RJ (2007) Biological control of wheat take-all disease: I-Characterization of antagonistic bacteria from diverse soils toward Gaeumannomyces graminis var. tritici. Tunisian Journal of Plant Protection 2: 23-34.
Ongena M, Jacques P (2007)Bacillus lipopeptides: versatile weapons for plant disease control. Trends Microbiology 16: 115-125.
Prigent-Combaret C, Vidal O, Dorel C, Lejeune P (1999) Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli. Journal of Bacteriology 181: 5993-6002.
Pyoung K, Ryu J, Kim YH, Chi WT (2010) Production of biosurfactant lipopeptides Iturin A, Fengycin, and Surfactin a from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides. Journal of Microbiology and Biotechnology 20: 138-145.
Sadekuzzaman M, Yang S, Mizan MFR, Ha SD (2015) Current and recent advanced strategies for combating biofilms. Comprehensive Reviews in Food Science and Food Safety 14: 491-509.
Sauer K (2003) The genomics and proteomics of biofilm formation. Available on line at: http://genomebiology.com.
Stanley NR, Britton RA, Grossman AD, Lazazzera BA (2003) Identification of catabolite repression as a physiological regulator of biofilm formation by Bacillus subtilis by use of DNA microarrays. Journal of Bacteriology 185: 1951-1957.
Timmusk S, Paalme V, Lagercratz U, Nevo E (2007)Detection and quantification of plant drought tolerance enhancing bacterium Paenibacillus polymyxa in the rhizosphere of wild barley (Hordeum spontaneum) with real-time PCR. Journal of Applied Microbiology 107: 736-745.
Ul Hassan T, Bano A (2015) The stimulatory effects of L-tryptophan and plant growth promoting rhizobacteria (PGPR) on soil health and physiology of wheat. Journal of Soil Science and Plant Nutrition 15: 190-201.
Vejan P, Abdullah R, Khadiran T, Ismail S, Nasrulhaq Boyce A (2016) Role of plant growth promoting rhizobacteria in agricultural sustainability-a review. Molecules 21: 573; Doi: 10.3390/molecules21050573.
Weller DM, Cook RJ (1983) Suppression of take-all of wheat by seed treatment with fluorescent pseudomonades. Phytopathology 73: 463-469.
Yan Z, Reddy MS, Kloepper JW (2003) Survival and colonization of rhizobacteria in a tomato transplant system. Canadian Journal of Microbiology 49: 383-389.
Yaryura PM, Leo M, Correa, OS, Kerber NL, Pucheu NL, García AF (2008) Assessment of the role of chemotaxis and biofilm formation as requirements for colonization of roots and seeds of soybean plants by Bacillus amyloliquefaciens BNM339. Current Microbiology 56: 625-632.
Zahid M, KaleemAbbasi M, Hameed S, Rahim N (2015) Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving  growth and nutrient contents of maize (Zea mays L.). Frontiers in Microbiology 6: 207; Doi: 10.3389/fmicb.2015.00207.
Zeriouh H, de Vicente A, Pérez-García A, Romero D (2014)Surfactin triggers biofilm formation of Bacillus subtilis in melon phylloplane and contributes to the biocontrol activity. Environmental Microbiology 16: 2196-2211.

Files

Share

How to cite

Statistics