Efficacy of the volatile organic compounds of Trichoderma asperellum in control of Botrytis cinerea, the causal agent of strawberry gray mold

Document Type : Complete paper

Authors

1 Ph. D. Student, Department of Plant Protection, College of Agriculture, Bu-Ali Sina University, Hamedan, Iran

2 Associate Professor, Department of Plant Protection, College of Agriculture, Bu-Ali Sina University, Hamedan, Iran

3 Assistant Professor, Department of Agronomy and P lant Breeding, College of Agriculture and Natural Recourses, Razi University, Kermanshah, Iran

Abstract

The present study was conducted to determine the efficacy of Trichoderma asperellum Volatile Organic Compounds (VOCs) in suppression of mycelial growth and conidial germination of Botrytis cinerea and also in control of Botrytis fruit rot of strawberry. Significant reduction in colony diameter, percent of conidial germination and length of germ tubes of B. cinerea was observed in treatments exposed to VOCs of 5-days old T. asperellum compared control. The incidence and severity of Botrytis fruit rot of strawberry was also significantly reduced by exposure to the VOCs of 5-day old T. asperellum cultures. VOCs profile of T. asperellum was identified using solid phase microextraction coupled to gas chromatography–mass spectrometry (SPME-GC-MS) technique. Six compounds out of 34 VOCs identified in this including isobutyric acid, 1,3,5,7-cyclooctatetraene, dimethyl sulfide, para-menta-6,8,dien-2-ol-acetate, phenyl ethyl alcohol and 1-butanol, 3-methyl-, acetate were the most abundant. Synthetic compounds of isobutyric acid and dimethyl sulfide were the most effective compounds among these compounds in suppression of mycelial growth and conidial germination of B. cinerea with IC50 of 26.5 and 10 μl/ liter, respectively.

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BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 5, Issue 2 - Serial Number 2
Autumn & Winter
December 2016
Pages 247-259

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