Investigation of Strawberry cultivars resistance against Botrytis cinerea and control effect of Anise essence and Iprodione-Carbendazim on it

Document Type : Complete paper

Authors

1 University of Agricultural and Natural Resources SARI

2 Sari Agricultural Sciences and Natural Resources University

3 Director of central plant protection clinic- Babolsar

Abstract

The Botrytis cinerea is one of the most important agents of Strawberry fruit rot. In order to identify the pathogenicity potential of the isolates on fruits and the antifungal effect of Anise essence on this fungus, forty one isolates of B. cinerea was collected from different areas of Mazandaran province on strawberry showing symptoms of gray rot on fruits. Following the purification of the fungus, pathogenicity test was done on Comarosa, Parus and Gilasi varieties of strawberries. Morphological identification of isolates was done in regard to their vegetative characteristics. Molecular identification carried out using specific primer C729 and common primers ITS4 and ITS5. Inhibition rate of fungal growth was recorded by Anise oil and Iprodione- Carbendazim fungicide. The results based on morphological characteristics showed that all isolates belonge to Botrytis cinerea. Identification of isolates using specific primer C729 showed that all of them had band of 700 bp. Based on disease severity on three strawberry cultivars; isolates were graded into seven groups. The ITS region of ribosomal DNA of each group was amplified using ITS4 and ITS5 primers. After sequencing and alignment, the obtained sequences were compared with the existing sequences in Gene Bank. The results showed there is high genetic diversity among the isolates, so that and they classified into five groups with 80% similarity. The result of in vitro evaluation of fungicide and Anise essence effect, showed that with increasing of Anise oil concentration, antifungal activity is also increasing against B. cinerea. Iprodione- Carnbendazim fungicide could inhibit the growth of isolates on a culture medium.

Keywords

Main Subjects

References

Abbott WS (1925) A method of computing the effectiveness of an insecticide. Journal of Economic Entomolgy 18: 265-267.
Anthony S, Abeywickrama K, Wilson WS (2003) The effect of spraying essential oils of Cymbopogon nardus, Cymbopogon flexuosus and Ocimum basilicum on post-harvest diseases and storage life of Embul banana. Journal of Horticultural Science and Biotechnology 78: 780-785.
Barnett HL, Hunter BB (1998) Illustrated genera of imperfect fungi. 4th ed., APS Press, USA.
Beever RE, Weeds PL (2004) Taxonomy and genetic variation of Botrytis and Botryotinia. Pp. 29-52 In: Elad Y, Williamson B, Tudzynski P and Delen N (eds) Botrytis: Biology, pathology and controls. Kluwer Academic Publisher, Netherland.
Bouchra C, Achouri M, Idrissi-Hassani LM, Hmamuchi M (2003) Chemical composition and antifungal activity of essential oils of seven Morcoccan labiatea against Botrytis cinerea Per: Fr. Journal of Ethnopharmacology 89: 165-169.
Capdeville GD, Maffia LA, Finger FL, Batista UG (2004) Pre-harvest calcium sulfate applications affect vase life and severity of gray mold in cut roses. Scientia Horticulturae 103: 329-338.
Chebli B, Hmamuchi M, Achouri M, Idrissi-Hassani LM (2004) Composition and in vitro fungitoxic activity of 19 essential oils against two post-harvest pathogens. Journal of Essential Oil Research 16: 507-511.
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
Elad Y, Williamson B, Tudzynski P, Delen N (2007) Botrytis: Biology, pathology and control. Published by Springer, Netherlands.
Ellis MB (1971) Dematiaceus, Hyphomycetes. Common Wealth Mycological Institute 178-184.
Faretra F, Antonacci E (1987) Production of apothecia of Botryotinia fuckeliana (de Bary) Whetz. under controlled environmental conditions. Phytopathologia Mediterranea 26: 29-35.
Farzaneh M, Ahmadzadeh M, Hadian J, Sharifi-Tehrani A (2006) Chemical composition and antifungal activity of the essential oils of three species of Artemisia on some soil-borne phytopathogens. Communications in Agricultural and Applied Biological Sciences 71(3b): 1327-1333.
Grindle M (1979) Phenotypic differences between natural and induced variants of Botrytis cinerea. Journal of General Microbiology 111: 109-120.
Hancock JF (1999) Strawberries (Crop production science in horticulture. Cab international, Wallingford, UK.
Hennebert GL (1973) Botrytis and Botrytis- like genera. Persoonia 7: 183-204.
Holst-Jensen A, Vaage M, Schumacher T (1998) An approximation to the phylogeny of Sclerotinia and related genera. Nordic Journal of Botany 18: 705-719.
Huang R, Li GQ, Zhang J, Yang L, Che HJ, Jiang DH, Huang HC (2011) Control of postharvest Botrytis fruit rot of strawberry by volatile organic compounds of Candida intermedia. Phtopathology 101(7): 859-869.
Jarvis WR (1977) Botryotinia and botrytis species: Taxonomy, physiology and pathogenicity. Research Branch, Canada Department of Agriculture, Ottawa, Canada.
Khazaeli P, Zamanizadeh H, Morid B, Bayat H (2010) Morphological and molecular identification of Botrytis cinerea causal agent of gray mold in rose greenhouse in central region of Iran. International Journal of Agricultural Science and Research 1: 19-24.
Kulakiotu EK, Thanassoulopoulos CC, Sfakiotakis EM (2004) Biological control of Botrytis cinerea by volatiles of “Isabella” grapes. Phytopathology 94: 924-931.
Milena C, Silva E (2005) Differences in the initial events of infection of Botrytis cinerea strains isolated from tomato and grape. Mycologia 97(2): 485-492.
Mirzaei S, Mohammadi Goltapeh E, Shams-bakhsh M (2008) Identification of Botrytis spp. on plants grown in Iran. Phytopathology 156: 21-28.
Ozan M (2003) Antifungal effects of some Turkish spice essential oils on Aspergillus niger and Botrytis cinerea growth. Agrimedia GmbH 8: 173-175.
Peighami-Ashnaei S, Farzaneh M, Hadian J, Sharifi-Tehrani A, Ghorbanpoor M (2007) Evaluation of antifungal activity of some plant essential oils against the gray mold of apple caused by Botrytis cinerea. Agricultural Research: Water, Soil and Plant in Agricultural 7(3): 1-10. (in Persian)
Plaza P, Torres R, Usall G, Lamarca N, Vinasa I (2004) Evaluation of the potential of commertial post-harvest application of essential oils to control citrus decay. Journal of Horticultural Science and Biotechnology 79: 935-940.
Rakhshani-Zabol E, Taheri A (2006) Principles of agricultural toxicology (Vol. II) fungicides, bactericides and nematicides biological Publishers of Farhnge Jame, Iran. (in Persian)
Rigotti S, Gindro K, Richter H, Viret O (2002) Charactrization of molecular marker for specific and sensitive detection of Botrytis cinerea Pers.: Fr. in strawberry (Fragaria ananassa Duch.) using PCR. FEMS Microbiology Letters 209: 169-174.
Saghi-Younesi B, Solimani MJ, Zafari D, Bahramnejad B (2014) Identification and study of genetic diversity of Botrytis cinerea isolates caused Strawberry grey mold in Kurdistan province. Biothechnology in Agricultural 13(2): 1-10. (In Persian)
Staats M, Baarlen PV, Van Kan JAL (2005) Molecular phylogeny of the plant pathogenic genus Botrytisand the evolution of host specificity. Molecular Biology and Evolution 22: 333-346.
Utkhede RS, Mathur S (2002) Biological control of stem canker of greenhouse tomatoes caused by Botrytis cinerea. Canadian Journal of Microbiology 48: 550-554.
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (ed.), PCR Protocols: A guide to Methods and Applications, Academic Press: San Diego, U.S.A. pp. 315-322.
Zadehdabagh Gh, Rokni N, Kiani S, Mohammadi Goltapeh E, Danesh RY (2010) Evaluation of Botrytis cinerea isolates for virulence on rose cut flowers in greenhouse condition in Iran. Journal of Agricultural Technology 6(3): 525-532.

Files

Share

How to cite

Statistics