Evaluation of Rhizoctoni solani control in cotton by Pseudomonas fluorescens isolates

Document Type : Complete paper

Authors

1 M. Sc. Student, Plant Protection, Department of Plant Protection, Azarbaijani Shahid Madani University

2 Assistant Professor, Department of Plant Protection, Azarbaijani Shahid Madani University

3 Assistant Professor, Department of Agronomy and Plant Breeding, Azarbaijani Shahid Madani University

Abstract

Plant growth-promoting rhizobacteria (PGPR) is an alternative to chemical pesticides against harmful microorganisms especially soil-borne diseases. Rhizoctonia solani is one of the most important soil-borne fungal pathogens that causes serious damages to important crops. In this study, to screen successful Pseudomonas bacterial isolates, the inhibition of pathogen growth was evaluated in-vitro. Moreover, isolates were screened based on the ability to auxin and ACC-deaminase production, phosphorus solubilization, siderophore production and possession of PhlD gene. From isolates studied for the extent of inhibition, 20 isolates were selected for further studies, among them, the isolates 33, 139 and 137 showed the highest rate of P solubility and ability to produce auxin. There was a correlation between the phosphorus solubility and pH reduction. Six isolates were selected to evaluate their capability for ACC-deaminase enzyme production based on the higher auxin production and better in-vitro inhibitory effect on the growth of Rhizoctoniasolani. All of the six strains appeared to have produce ACC-deaminase enzyme. PhlD gene that plays a key role in 2,4-diacetylphloroglucinol antibiotic biosynthesis pathway, was detected in the isolates 153, 139, 137, CHA0 and 58A. According to the characteristics, five isolates were selected for greenhouse experiments. In greenhouse experiments, all five isolates controlled disease significantly, and among them, the 137 isolate showed the highest control level

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References

Abbas-Zadeh P, Saleh-Rastin N, Asadi-Rahmani H, Khavazi K, Soltani A, Shoary-Nejati AR,Mohammad Miransari (2010) Plant growth-promoting activities of fluorescent pseudomonads, isolated from the Iranian soils. Acta Physiologae Plantarum 32: 281-288.
Ahmad F, Ahmad I, Khan MS (2008) Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiologycal Research 163: 173-181.
Amico ED, Cavalca L, Andreoni V (2005) Analysis of rhizobacterial communities in perennial Graminaceae from polluted water meadow soil, and screening of metal-resistant, potentially plant growth-promoting bacteria. FEMS Microbiology Ecology 52: 153-162.
Azaddysfani F, Rohani H, Falahati Rastgar M, Mehdikhani magdom A (1390) Cotton immune responses to Trichoderma species and its effect on the control of damping off caused by Rhizoctonia solani. Plant Protection 27: 1-10. (In Persian)
Bashan Y (1994) Symptom expression and ethylene production in leaf blight of cotton caused by Alternaria macrospora and Alternaria alternaria taalone and combined. Botany 72: 1574-1579.
Belimov AA, Dodd IC, Safronova VI, Hontzeas N, Davies WJ (2007) Pseudomonas brassicacearum strain Am3 containing 1- aminocyclopropane-1-carboxylate deaminase can show both pathogenic and growth-promoting properties in its interactionwith tomato. Experimental Botany 58(6): 1485-95.
Belimov AA, Hotzeas N, Safronova VI, Demchinskaya SV, Piluzza G, Bullitta S, Glick BR (2005) Cadmium-tolerant plant growth- promoting bacteria associated with the roots of Indian mustard (Brassica juncea L. Czern.). Soil Biology and Biochemistry 37: 241-250.
Bhattacharyya PN and Jha DK (2012) Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. Microbiol Biotechnology 28: 1327-1350.
Castaneola GC, Munoc TJJ, Videa JRP (2005) A spectrophotometric method to determinethe siderophore production b strain of fluorescent Pseudomonas in the presence of copper and iron. Microchemical 81: 35-40.
Dey R, Pal KK, Bhatt DM, Chauhan SM (2004) Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiologycal Research 159: 371-394.
Duijff BJ, Meijer JW, Bakker PAHM, Schippers B (1993) Siderophore-mediated competition for iron and induced resistance in the suppression of Fusarium wilt of carnation by fluorescent Pseudomonads spp. Plant Pathology. 99: 277-289.
Fiddaman PJ, Rossall K (1994) Effect of substrate on the production of antifungal volatiles from Bacillus subtilis. J Applied Bacteriology 79: 395-405.
Glass ADM (1989) Plant nutrition: an introduction to current concepts. Jones and Bartlett Publishers, Boston, p 234.
Glick BR, Jacobson CB, Schwarze MM, Pasternak JJ (1994) 1-Aminocyclopropane-1 carboxylic acid deaminase mutants of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 do not stimulate canola root elongation. Microbiology 40: 911-915.
Hagedorn C, Gould WD, Bardinelli TR (1998) Rhizobacteria of cotton and their repression of seedling Disease pathogens. Applied and Environmental Microbiology 55: 2793-2797.
Harrison  LA, Letendre L, Kovacevich P, Pierson  FA, Weller DM (1993) Purification of an antibiotic effective against Gaeumannomyce graminis var. tritici produced by a biocontrol agent, Pseudomonas aureofaciens. Soil Biogy and Biochemistry 25, 215-221.
Han J, Sun L, Dong X, Cai Z, Sun X, Yang H, Wang Y, Song W (2005) Characterization of a novel plant growth-promoting bacteria strain Delftia tsuruhatensis HR4 both as a diazotroph and a potential biocontrol agent against various plant pathogens. Systematic and Applied Microbiology 28(1): 66-76.
Hilali A, Przvost D, Broughton WJ, Antoun H (2000) Potential use of Rhizobium leguminosarum bv. trifolii as plant growth promoting rhizobacteria with wheat. Abstract, 17th North American Conference on Symbiotic Nitrogen Fixation, Laval University, Quebec, Canada.
Hillocks RJ (1992) Cotton Diseases. C. A. B. International Wallingford. UF. 415 pp.
Howell CR, Stipanovic RD (1979) Control of Rhizoctonia solani on cotton seedlings with Pseudomonas fluorescens and with an antibiotic produced by the bacteri um. Phytopathology 69: 480-482.
Jeon JS, Lee SS, Kim HY, Ahn TS and Song HG (2003) Plant growth promotion in soil by some inoculated microorganism. Microbiology 41: 271-276.
Khakipour N, Khavazi K, Mojallali H, Pazira E, Asadirahmani H (2008) Production of auxin hormone by fluorescent pseudomonads. Agricultural and Environmental Science 4: 687-692.
Kloepper J, Lifshitz WR, Schroth MN (1988) Pseudomonas inoculants to benefit plant production. Animal and Plant Sciences 60-64.
Kloepper JW, Gutierrez-Estrada A, Mclnroy JA (2007) Photoperiod regulates elicitation of growth promotion but not induced resistance by plant growth-promoting rhizobacteria. Microbiology 53(2): 159-167.
Lemanceau P, Robin A, Mazurier S, Vansuyt G (2006) Implication of Pyoverdines in the Interactions of Fluorescent Pseudomonads with Soil Microflora and Plant in the Rhizosphere. Microbal siderophores Pp. 165-192.
Loper JE, Henkels MD (1999) Utilization of heterologos siderophores enhances levels of iron available to Pseudomonas putida in the rhizosphere. Applied and Environmental Microbiology 65: 5357-5363.
Pal KK, Tilak KVBR, Saxena AK, Dey R, Singh CS (2001) Suppression of maize root diseases caused by Macrophomina phaseolina, Fusarium moniliforme and Fusarium graminearum by plant growth promoting rhizobacteria. Microbiological Research 156: 209-223.
Pandey P, Kang SC, Maheshwari DK (2005) Isolation of endophytic plant growth promoting Burkholderia sp. MSSP from root nodules of Mimosa pudica. Current Science 89: 170-180.
Patten CL, Glick BR (2002) The role of bacterial indoleacetic acid in the development of the host plant root system. Applied and Environmental Microbiology 68: 3795-3801.
Ping  L, Boland W (2004) Signals from the underground: bacterial volatiles promote  growth in Arabidopsis.Trends in plant Science 9: 263-266.
Rashid MS, Khalil N, Ayub S, Alam S, Latif F (2004) Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions. Biology Sciences 7: 187-196.
Richardson AE, Barea JM, McNeill AM, Prigent-Combaret C (2009) Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant and Soil 321: 305-339.
Saleem M, Arshad M, Hussain S, Bhatti AS (2007) Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture. Microbiol Biotechnology 34: 635-648.
Sharifi R, Ahmadzadeh M, Sharifi Tehrani A, Fallahzadeh V, (1387) Competition for Iron Uptake by Fluorescent Pseudomonads to Control of Rhizoctonia Solani Kuhn Causing Agent of Bean Damping-Off Disease. Plant Protection 22: 183-195. (In Persian)
Shirzad A, Valizadeh Kamran R, Ahmadabadi M (1388) Comparison of Fluorescent Pseudomonads DNA extraction methods for PCR. Sixth National Congress of Biotechnology. (In Persian)
Shirzad A, Fallahzadeh-Mamagani V, Pazhouhandeh M (2012) Antagonistic potential of Fluorescent pseudomonas and control of crown and root root of cucumber caused by Phythophtora drechsleri. Plant Pathology 28(1): 1-9.
Solymani Monfared P, Kazemi Tabar K, Tohidfar M, Ebrahimi T (1391) Identification and cloning of the gene coding for antibiotic DAPG of Fluorescent Pseudomonads UTP-F68 gene. J Genetic of Third Millenary 10(1): 2649-2655. (In Persian)
Spaepen S, Vanderleyden J, Remans R (2007) Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS microbiology Reviews. pp 1-24.
Tao G, Tian S, Cai M, Xie G (2008) Phosphate solubilizing and mineralizing abilities of bacteria isolated from soils. Pedosphere 18: 515-523.
Tjamos EC, Tsitsigiannis DI, Tjamos SE, Antoniou, PP, Katinakis P (2004) Selection and screening of endorhizosphere bacteria from solarized soil as biocontrol agents against verticillium dahlia of solanaceous hosts. Plant Pathology 110: 35-44.
Thomashow LS, Bonsall RF, Weller DM (2002) Antibiotic production by soil and rhizosphere microbes in situ. In Manual of Environmental Microbiology 99: 164-6430.
Thomashow LS, Weller DM (1995) Current concepts in the use of introduced bacteria for biological disease control. Plant-microbe interactions 1: 187-235.
Wang C, Ramette A, Punjasamarnwong P, Zala M, Natsch A, Moenne-Loccoz Y, Defago G (2001) Cosmopolitan distribution of phlD-containing dicotyledonous crop-associated biocontrol pseudomonads of worldwide origin. FEMS Microbiolology Ecology 37: 105-116.
Weller DM (1988) Biological control of soilborne Plant patholgens in the Rhizosphere with bacteria. Annual review of Phytopathology 26: 379-407.
Weller DM, Cooke RJ (1983) Suppression of take all of wheat by seed treatment with fluorescent pseudomonas. Phytopathology 73(3): 463-4.
BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 4, Issue 1 - Serial Number 1
Spring & Summer
April 2015
Pages 23-36

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