The use of silicon on systemic resistance induction against fusarium head blight disease of wheat

Document Type : Complete paper

Authors

1 MSc student of plant pathology, department of plant protection, University of Zabol, Iran

2 Assistant professor,department of plant pathology, and institute of plant biotechnology, University of Zabol, Iran

3 Assistant professor, department of biology, faculty of Basic Sciences, University of Zabol, Iran

4 Assistant professor, faculty of plant pathology, University of Zabol, Iran

Abstract

In this study the effect of different concentrations of silicon( 0, 2 , 4 and 6 mM, on expression of some genes involved in resistance and activity of several antioxidan enzymes was investigated: The plants was treated in flowering stage via soil solution. The treated plants were inoculated with 106 spore suspended in 1 mL by injection into the lower part of the spikelets and then were harvested at 0, 24, 72 and 120 h after inoculation for meausering enzymatic activity of gene expression analysis. The results showed that disease severity was reduced in treated plants compared to the control. Biochemical analysis showed that activity of peroxidase and polyphenol oxidase enzymes at all concentrations of silicon was increased significantly copared to the control . Gene expression analysis showed that expression level of beta-1, 3-glucanase and oxalate oxidase genes induced plants with silicon concentration of 6mmol/l were increased at 120h time point after inoculation. Overal, The results of this research indicate that silicon could increase plant resistance against pathogenic fungi by induction of induced systemic resistance.

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Main Subjects

References

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BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES
Volume 3, Issue 2 - Serial Number 2
October 2014
Pages 127-137

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