پردیس کشاورزی و منابع طبیعی دانشگاه تهران موسسه تحقیقات کنترل بیولوژیک آفات و بیماریهای گیاهی BIOLOGICAL CONTROL OF PESTS AND PLANT DISEASES 2322-2883 7 1 2018 03 21 Optimization of antifungal and insecticidal effects of garden thyme (Thymus vulgaris L.) essential oil through response surface methodology Optimization of antifungal and insecticidal effects of garden thyme (Thymus vulgaris L.) essential oil through response surface methodology 9 19 67163 10.22059/jbioc.2018.252650.223 FA Asgar Ebadollahi Moghan College Of Agriculture & Natural Resources, University of Mohaghegh Ardabili, Iran Ebarahim Taghinezhad Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. Mahdi Davari Department of Plant Protection, College of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran. Journal Article 2018 02 14 In the present study, optimization and modeling of the mycelial growth inhibitory and fumigant toxicity of Thymus vulgaris essential oil were investigated using Response Surface Methodology (RSM) against the pathogenic fungi Fusarium graminearum and Botrytis cinerea‌ and the lesser grain beetle (Rhyzopertha dominica), respectively. The chemical composition of this essential oil was also assessed through Gas Chromatography-Mass Spectrometry (GC-MS) and carvacrol (27.99%), thymol (12.76%), and geraniol (8.91%) were identified as its main components. Mycelial growth inhibition of pathogenic fungi and insect pest mortality was significantly enhanced with increasing of the essential oil concentrations and the exposure times. Optimum conditions to achieve maximum mycelial growth inhibitory (88.67%) were evaluated as time of 88.59 h and the concentration of 486.66 ppm. Also, a time of 74.01-h and the concentration of 552.96 ppm were caused the maximum growth inhibitory of B. cinerea (94.43%). The concentration of 43.10 µl/l and 60 h exposure time were estimated as optimum conditions for achieving 72.23% mortality of R. dominica. Results demonstrated that the essential oil of T. vulgaris has a high potential for management of F. graminearum and B. cinerea and the lesser grain beetle. In the present study, optimization and modeling of the mycelial growth inhibitory and fumigant toxicity of Thymus vulgaris essential oil were investigated using Response Surface Methodology (RSM) against the pathogenic fungi Fusarium graminearum and Botrytis cinerea‌ and the lesser grain beetle (Rhyzopertha dominica), respectively. The chemical composition of this essential oil was also assessed through Gas Chromatography-Mass Spectrometry (GC-MS) and carvacrol (27.99%), thymol (12.76%), and geraniol (8.91%) were identified as its main components. Mycelial growth inhibition of pathogenic fungi and insect pest mortality was significantly enhanced with increasing of the essential oil concentrations and the exposure times. Optimum conditions to achieve maximum mycelial growth inhibitory (88.67%) were evaluated as time of 88.59 h and the concentration of 486.66 ppm. Also, a time of 74.01-h and the concentration of 552.96 ppm were caused the maximum growth inhibitory of B. cinerea (94.43%). The concentration of 43.10 µl/l and 60 h exposure time were estimated as optimum conditions for achieving 72.23% mortality of R. dominica. Results demonstrated that the essential oil of T. vulgaris has a high potential for management of F. graminearum and B. cinerea and the lesser grain beetle. https://jbiocontrol.ut.ac.ir/article_67163_14c2fc12a76b2f8ab0087e40040c2b08.pdf